TY - JOUR
T1 - Evidence for sequestration of polyglutamine inclusions by Drosophila myeloid leukemia factor
AU - Kim, Woo Yang
AU - Fayazi, Zahra
AU - Bao, Xiankun
AU - Higgins, Dennis
AU - Kazemi-Esfarjani, Parsa
N1 - Funding Information:
This investigation was supported by National Science Foundation (NSF) (No. IBN0121210) to D.H., and by the National Institutes of Health (NIH)/NINDS (No. NS42162) and Howard Hughes Medical Institute Biomedical Research Support Program (No. 53000261) to P.K.-E.
PY - 2005/8
Y1 - 2005/8
N2 - Intracellular inclusions of abnormally long polyglutamine tracts and neurotoxicity are the hallmarks of several hereditary neurodegenerative disorders, including Huntington's disease (HD). In Drosophila melanogaster, dMLF, an ortholog of human myeloid leukemia factors, hMLF1 and hMLF2, suppressed polyglutamine toxicity and colocalized with the inclusions. In transfected primary rat neuronal cultures, dMLF and its orthologs reduced the morphological phenotypes and inclusions. Furthermore, dMLF reduced the recruitment of CBP and Hsp70 into the inclusions, both of which are among many essential proteins apparently trapped in the inclusions. These data suggest that a possible mechanism of suppression by dMLF is via the sequestration of polyglutamine oligomers or inclusions.
AB - Intracellular inclusions of abnormally long polyglutamine tracts and neurotoxicity are the hallmarks of several hereditary neurodegenerative disorders, including Huntington's disease (HD). In Drosophila melanogaster, dMLF, an ortholog of human myeloid leukemia factors, hMLF1 and hMLF2, suppressed polyglutamine toxicity and colocalized with the inclusions. In transfected primary rat neuronal cultures, dMLF and its orthologs reduced the morphological phenotypes and inclusions. Furthermore, dMLF reduced the recruitment of CBP and Hsp70 into the inclusions, both of which are among many essential proteins apparently trapped in the inclusions. These data suggest that a possible mechanism of suppression by dMLF is via the sequestration of polyglutamine oligomers or inclusions.
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U2 - 10.1016/j.mcn.2005.04.005
DO - 10.1016/j.mcn.2005.04.005
M3 - Article
C2 - 15936212
AN - SCOPUS:22144471491
SN - 1044-7431
VL - 29
SP - 536
EP - 544
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 4
ER -