Evidence that acetaldehyde irreversibly impairs glycoprotein metabolism in liver slices

M. F. Sorrel; D. J. Tuma; A. J. Barak

doi:10.1016/s0016-5085(19)31872-4

Evidence that acetaldehyde irreversibly impairs glycoprotein metabolism in liver slices

M. F. Sorrel, D. J. Tuma, A. J. Barak

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

When liver slices were incubated for 1 hr in a medium containing either acetaldehyde or ethanol and then switched to a fresh medium free from acetaldehyde but containing the appropriate radioactive precursors and reincubated for 3 additional hr, a decreased incorporation of both [¹⁴C]glucosamine and [¹⁴C]leucine into hepatocellular and secretory proteins was observed. Further studies were carried out comparing the inhibitory effects of ethanol and acetaldehyde on drug metabolism. Higher concentrations of ethanol were necessary to impair aniline hydroxylation when compared to its effect on glycoprotein metabolism. Acetaldehyde did not affect aniline metabolism, suggesting some specificity for its effect on intracellular metabolic functions. The results show that the acetaldehyde-induced inhibition of glycoprotein synthesis is irreversible under these short term experimental conditions and is not dependent on the physical presence of acetaldehyde in the incubation medium.

Original language	English (US)
Pages (from-to)	1138-1141
Number of pages	4
Journal	Gastroenterology
Volume	73
Issue number	5
DOIs	https://doi.org/10.1016/s0016-5085(19)31872-4
State	Published - 1977
Externally published	Yes

ASJC Scopus subject areas

Hepatology
Gastroenterology

Access to Document

10.1016/s0016-5085(19)31872-4

Cite this

@article{1ca1496ed08a402399395269a0620037,

title = "Evidence that acetaldehyde irreversibly impairs glycoprotein metabolism in liver slices",

abstract = "When liver slices were incubated for 1 hr in a medium containing either acetaldehyde or ethanol and then switched to a fresh medium free from acetaldehyde but containing the appropriate radioactive precursors and reincubated for 3 additional hr, a decreased incorporation of both [14C]glucosamine and [14C]leucine into hepatocellular and secretory proteins was observed. Further studies were carried out comparing the inhibitory effects of ethanol and acetaldehyde on drug metabolism. Higher concentrations of ethanol were necessary to impair aniline hydroxylation when compared to its effect on glycoprotein metabolism. Acetaldehyde did not affect aniline metabolism, suggesting some specificity for its effect on intracellular metabolic functions. The results show that the acetaldehyde-induced inhibition of glycoprotein synthesis is irreversible under these short term experimental conditions and is not dependent on the physical presence of acetaldehyde in the incubation medium.",

author = "Sorrel, {M. F.} and Tuma, {D. J.} and Barak, {A. J.}",

year = "1977",

doi = "10.1016/s0016-5085(19)31872-4",

language = "English (US)",

volume = "73",

pages = "1138--1141",

journal = "Gastroenterology",

issn = "0016-5085",

publisher = "W.B. Saunders Ltd",

number = "5",

}

TY - JOUR

T1 - Evidence that acetaldehyde irreversibly impairs glycoprotein metabolism in liver slices

AU - Sorrel, M. F.

AU - Tuma, D. J.

AU - Barak, A. J.

PY - 1977

Y1 - 1977

N2 - When liver slices were incubated for 1 hr in a medium containing either acetaldehyde or ethanol and then switched to a fresh medium free from acetaldehyde but containing the appropriate radioactive precursors and reincubated for 3 additional hr, a decreased incorporation of both [14C]glucosamine and [14C]leucine into hepatocellular and secretory proteins was observed. Further studies were carried out comparing the inhibitory effects of ethanol and acetaldehyde on drug metabolism. Higher concentrations of ethanol were necessary to impair aniline hydroxylation when compared to its effect on glycoprotein metabolism. Acetaldehyde did not affect aniline metabolism, suggesting some specificity for its effect on intracellular metabolic functions. The results show that the acetaldehyde-induced inhibition of glycoprotein synthesis is irreversible under these short term experimental conditions and is not dependent on the physical presence of acetaldehyde in the incubation medium.

AB - When liver slices were incubated for 1 hr in a medium containing either acetaldehyde or ethanol and then switched to a fresh medium free from acetaldehyde but containing the appropriate radioactive precursors and reincubated for 3 additional hr, a decreased incorporation of both [14C]glucosamine and [14C]leucine into hepatocellular and secretory proteins was observed. Further studies were carried out comparing the inhibitory effects of ethanol and acetaldehyde on drug metabolism. Higher concentrations of ethanol were necessary to impair aniline hydroxylation when compared to its effect on glycoprotein metabolism. Acetaldehyde did not affect aniline metabolism, suggesting some specificity for its effect on intracellular metabolic functions. The results show that the acetaldehyde-induced inhibition of glycoprotein synthesis is irreversible under these short term experimental conditions and is not dependent on the physical presence of acetaldehyde in the incubation medium.

UR - http://www.scopus.com/inward/record.url?scp=0017662766&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017662766&partnerID=8YFLogxK

U2 - 10.1016/s0016-5085(19)31872-4

DO - 10.1016/s0016-5085(19)31872-4

M3 - Article

C2 - 908493

AN - SCOPUS:0017662766

VL - 73

SP - 1138

EP - 1141

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 5

ER -

Evidence that acetaldehyde irreversibly impairs glycoprotein metabolism in liver slices

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this