Evidence that certain peripheral anti-acetylcholine receptor antibodies do not interact with brain BuTX binding sites

Barbara J. Morley; Donald S. Dwyer; Priscilla F. Strang-Brown; Ronald J. Bradley; George E. Kemp

doi:10.1016/0006-8993(83)90474-2

Evidence that certain peripheral anti-acetylcholine receptor antibodies do not interact with brain BuTX binding sites

Barbara J. Morley, Donald S. Dwyer, Priscilla F. Strang-Brown, Ronald J. Bradley, George E. Kemp

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The cross-reactivity of rat brain α-bungarotoxin (BuTX) binding sites with peripheral anti-acetylcholine receptor (nAChR) polyclonal and monoclonal antibodies was investigated in Triton extracts of several brain areas and purified whole brain putative receptors using radioimmunoassays and IgG/CNBr-Sepharose 4B immunoaffinity chromatography. The effects of the chronic intracerebral administration of antibodies on [¹²⁵I]BuTX binding and food and water intake were also investigated. It was concluded that brain BuTX sites do not share antigenic determinants with peripheral nAChRs. Additionally, no evidence was found to support the suggestions that anti-nAChR antibodies affect food and water intake, or that chronic administration of antibodies alters [¹²⁵I]BuTX binding in the hypothalamus of rats.

Original language	English (US)
Pages (from-to)	109-116
Number of pages	8
Journal	Brain Research
Volume	262
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)90474-2
State	Published - Feb 28 1983

Keywords

anti-acetylcholine receptor antibodies
brain
α-bungarotoxin binding sites

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Evidence that certain peripheral anti-acetylcholine receptor antibodies do not interact with brain BuTX binding sites",

abstract = "The cross-reactivity of rat brain α-bungarotoxin (BuTX) binding sites with peripheral anti-acetylcholine receptor (nAChR) polyclonal and monoclonal antibodies was investigated in Triton extracts of several brain areas and purified whole brain putative receptors using radioimmunoassays and IgG/CNBr-Sepharose 4B immunoaffinity chromatography. The effects of the chronic intracerebral administration of antibodies on [125I]BuTX binding and food and water intake were also investigated. It was concluded that brain BuTX sites do not share antigenic determinants with peripheral nAChRs. Additionally, no evidence was found to support the suggestions that anti-nAChR antibodies affect food and water intake, or that chronic administration of antibodies alters [125I]BuTX binding in the hypothalamus of rats.",

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AU - Morley, Barbara J.

AU - Dwyer, Donald S.

AU - Strang-Brown, Priscilla F.

AU - Bradley, Ronald J.

AU - Kemp, George E.

PY - 1983/2/28

Y1 - 1983/2/28

N2 - The cross-reactivity of rat brain α-bungarotoxin (BuTX) binding sites with peripheral anti-acetylcholine receptor (nAChR) polyclonal and monoclonal antibodies was investigated in Triton extracts of several brain areas and purified whole brain putative receptors using radioimmunoassays and IgG/CNBr-Sepharose 4B immunoaffinity chromatography. The effects of the chronic intracerebral administration of antibodies on [125I]BuTX binding and food and water intake were also investigated. It was concluded that brain BuTX sites do not share antigenic determinants with peripheral nAChRs. Additionally, no evidence was found to support the suggestions that anti-nAChR antibodies affect food and water intake, or that chronic administration of antibodies alters [125I]BuTX binding in the hypothalamus of rats.

AB - The cross-reactivity of rat brain α-bungarotoxin (BuTX) binding sites with peripheral anti-acetylcholine receptor (nAChR) polyclonal and monoclonal antibodies was investigated in Triton extracts of several brain areas and purified whole brain putative receptors using radioimmunoassays and IgG/CNBr-Sepharose 4B immunoaffinity chromatography. The effects of the chronic intracerebral administration of antibodies on [125I]BuTX binding and food and water intake were also investigated. It was concluded that brain BuTX sites do not share antigenic determinants with peripheral nAChRs. Additionally, no evidence was found to support the suggestions that anti-nAChR antibodies affect food and water intake, or that chronic administration of antibodies alters [125I]BuTX binding in the hypothalamus of rats.

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KW - α-bungarotoxin binding sites

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