Evidence that certain peripheral anti-acetylcholine receptor antibodies do not interact with brain BuTX binding sites

Barbara J. Morley; Donald S. Dwyer; Priscilla F. Strang-Brown; Ronald J. Bradley; George E. Kemp

doi:10.1016/0006-8993(83)90474-2

Evidence that certain peripheral anti-acetylcholine receptor antibodies do not interact with brain BuTX binding sites

Barbara J. Morley, Donald S. Dwyer, Priscilla F. Strang-Brown, Ronald J. Bradley, George E. Kemp

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The cross-reactivity of rat brain α-bungarotoxin (BuTX) binding sites with peripheral anti-acetylcholine receptor (nAChR) polyclonal and monoclonal antibodies was investigated in Triton extracts of several brain areas and purified whole brain putative receptors using radioimmunoassays and IgG/CNBr-Sepharose 4B immunoaffinity chromatography. The effects of the chronic intracerebral administration of antibodies on [¹²⁵I]BuTX binding and food and water intake were also investigated. It was concluded that brain BuTX sites do not share antigenic determinants with peripheral nAChRs. Additionally, no evidence was found to support the suggestions that anti-nAChR antibodies affect food and water intake, or that chronic administration of antibodies alters [¹²⁵I]BuTX binding in the hypothalamus of rats.

Original language	English (US)
Pages (from-to)	109-116
Number of pages	8
Journal	Brain Research
Volume	262
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)90474-2
State	Published - Feb 28 1983

Keywords

anti-acetylcholine receptor antibodies
brain
α-bungarotoxin binding sites

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(83)90474-2

Cite this

@article{0079e4ab57754f409f2ff41870cd6bf3,

title = "Evidence that certain peripheral anti-acetylcholine receptor antibodies do not interact with brain BuTX binding sites",

abstract = "The cross-reactivity of rat brain α-bungarotoxin (BuTX) binding sites with peripheral anti-acetylcholine receptor (nAChR) polyclonal and monoclonal antibodies was investigated in Triton extracts of several brain areas and purified whole brain putative receptors using radioimmunoassays and IgG/CNBr-Sepharose 4B immunoaffinity chromatography. The effects of the chronic intracerebral administration of antibodies on [125I]BuTX binding and food and water intake were also investigated. It was concluded that brain BuTX sites do not share antigenic determinants with peripheral nAChRs. Additionally, no evidence was found to support the suggestions that anti-nAChR antibodies affect food and water intake, or that chronic administration of antibodies alters [125I]BuTX binding in the hypothalamus of rats.",

keywords = "anti-acetylcholine receptor antibodies, brain, α-bungarotoxin binding sites",

author = "Morley, {Barbara J.} and Dwyer, {Donald S.} and Strang-Brown, {Priscilla F.} and Bradley, {Ronald J.} and Kemp, {George E.}",

year = "1983",

month = feb,

day = "28",

doi = "10.1016/0006-8993(83)90474-2",

language = "English (US)",

volume = "262",

pages = "109--116",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Evidence that certain peripheral anti-acetylcholine receptor antibodies do not interact with brain BuTX binding sites

AU - Morley, Barbara J.

AU - Dwyer, Donald S.

AU - Strang-Brown, Priscilla F.

AU - Bradley, Ronald J.

AU - Kemp, George E.

PY - 1983/2/28

Y1 - 1983/2/28

N2 - The cross-reactivity of rat brain α-bungarotoxin (BuTX) binding sites with peripheral anti-acetylcholine receptor (nAChR) polyclonal and monoclonal antibodies was investigated in Triton extracts of several brain areas and purified whole brain putative receptors using radioimmunoassays and IgG/CNBr-Sepharose 4B immunoaffinity chromatography. The effects of the chronic intracerebral administration of antibodies on [125I]BuTX binding and food and water intake were also investigated. It was concluded that brain BuTX sites do not share antigenic determinants with peripheral nAChRs. Additionally, no evidence was found to support the suggestions that anti-nAChR antibodies affect food and water intake, or that chronic administration of antibodies alters [125I]BuTX binding in the hypothalamus of rats.

AB - The cross-reactivity of rat brain α-bungarotoxin (BuTX) binding sites with peripheral anti-acetylcholine receptor (nAChR) polyclonal and monoclonal antibodies was investigated in Triton extracts of several brain areas and purified whole brain putative receptors using radioimmunoassays and IgG/CNBr-Sepharose 4B immunoaffinity chromatography. The effects of the chronic intracerebral administration of antibodies on [125I]BuTX binding and food and water intake were also investigated. It was concluded that brain BuTX sites do not share antigenic determinants with peripheral nAChRs. Additionally, no evidence was found to support the suggestions that anti-nAChR antibodies affect food and water intake, or that chronic administration of antibodies alters [125I]BuTX binding in the hypothalamus of rats.

KW - anti-acetylcholine receptor antibodies

KW - brain

KW - α-bungarotoxin binding sites

UR - http://www.scopus.com/inward/record.url?scp=0020680848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020680848&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(83)90474-2

DO - 10.1016/0006-8993(83)90474-2

M3 - Article

C2 - 6831222

AN - SCOPUS:0020680848

SN - 0006-8993

VL - 262

SP - 109

EP - 116

JO - Brain Research

JF - Brain Research

IS - 1

ER -

Evidence that certain peripheral anti-acetylcholine receptor antibodies do not interact with brain BuTX binding sites

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this