Evolutionary aspects of urea utilization by fungi

Dhammika H.M.L.P. Navarathna, Steven D. Harris, David D. Roberts, Kenneth W. Nickerson

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The higher fungi exhibit a dichotomy with regard to urea utilization. The hemiascomycetes use urea amidolyase (DUR1,2), whereas all other higher fungi use the nickel-containing urease. Urea amidolyase is an energy-dependent biotin-containing enzyme. It likely arose before the Euascomycete/Hemiascomycete divergence c. 350 million years ago by insertion of an unknown gene into one copy of a duplicated methylcrotonyl CoA carboxylase (MccA). The dichotomy between urease and urea amidolyase coincides precisely with that for the Ni/Co transporter (Nic1p), which is present in the higher fungi that use urease and is absent in those that do not. We suggest that the selective advantage for urea amidolyase is that it allowed the hemiascomycetes to jettison all Ni 2+- and Co2+-dependent metabolisms and thus to have two fewer transition metals whose concentrations need to be regulated. Also, the absence of MccA in the hemiascomycetes coincides with and may explain their production of fusel alcohols.

Original languageEnglish (US)
Pages (from-to)209-213
Number of pages5
JournalFEMS Yeast Research
Volume10
Issue number2
DOIs
StatePublished - Mar 2010

Keywords

  • DUR1,2
  • Fusel alcohols
  • Methylcrotonyl CoA carboxylase
  • Ni-dependent enzymes
  • Urea amidolyase
  • Urease

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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