TY - JOUR
T1 - Evolutionary relationship of three mitten crabs (Eriocheir sp)revealed by mitogenome and 5S ribosomal DNA analysis
AU - Chen, Xiaowen
AU - Wang, Jun
AU - Huang, Lei
AU - Yue, Wucheng
AU - Zou, Jixing
AU - Yuan, Chunhong
AU - Lu, Guoqing
AU - Wang, Chenghui
N1 - Funding Information:
The authors would like to thank to Mr. Yongju Luo at Guangxi Fisheries Institute in China for sampling the Hepu mitten crab. This work was funded by the Shanghai Agriculture Applied Technology Development Program, China (Grant No. G2017-02-08-00-10-F00076 ), the Shanghai Mitten Crab Industry Technology System Project (Grant No. 2017–2021 ), Young teachers training Project of Shanghai Municipal Education Commission ( A1-2056-16-0026 ), the Shanghai Science and Technology Project (Grant No. 16391905300 , 13DZ2251800 ), and Doctoral Program of Shanghai Ocean University ( A2-0203-00-100315 ).
Publisher Copyright:
© 2017 Shanghai Ocean University
PY - 2017/11
Y1 - 2017/11
N2 - To reveal the evolutionary relationship of three mitten crabs (Eriocheir sinensis, E. hepuensis, and E. japonica), complete mitogenomes and nuclear 5S rDNA sequences were analyzed. Sequencing revealed that the mitogenomes analyzed shared conserved organization of the coding and non-coding regions but genetic variation was identified. Among the three mitten crabs distinct tandem repeats were identified in the mitochondrial D-loop region. The 5S gene (5S rDNA)sequence was highly conserved across the three species, whereas non-transcribed spacer (NTS)region exhibit high levels of variation including insertions, deletions and point mutations. Cluster analysis suggested that the three mitten crabs had their own independent 5S rDNA sequence variation and evolutionary pattern. Both mitogenome and 5S rDNA sequence analysis revealed significant genetic variation across the mitten crabs species. Phylogenetic analysis using mitogenome and 5S rDNA sequences demonstrated that E. japonica was relatively more distant from E. sinensis and E. hepuensis. This study extended our previous knowledge and confirmed that the three mitten crabs are likely to be genetically differentiated species. In addition, our study also provided insights into the conservation of pure natural resources of E. sinensis, an important aquaculture species.
AB - To reveal the evolutionary relationship of three mitten crabs (Eriocheir sinensis, E. hepuensis, and E. japonica), complete mitogenomes and nuclear 5S rDNA sequences were analyzed. Sequencing revealed that the mitogenomes analyzed shared conserved organization of the coding and non-coding regions but genetic variation was identified. Among the three mitten crabs distinct tandem repeats were identified in the mitochondrial D-loop region. The 5S gene (5S rDNA)sequence was highly conserved across the three species, whereas non-transcribed spacer (NTS)region exhibit high levels of variation including insertions, deletions and point mutations. Cluster analysis suggested that the three mitten crabs had their own independent 5S rDNA sequence variation and evolutionary pattern. Both mitogenome and 5S rDNA sequence analysis revealed significant genetic variation across the mitten crabs species. Phylogenetic analysis using mitogenome and 5S rDNA sequences demonstrated that E. japonica was relatively more distant from E. sinensis and E. hepuensis. This study extended our previous knowledge and confirmed that the three mitten crabs are likely to be genetically differentiated species. In addition, our study also provided insights into the conservation of pure natural resources of E. sinensis, an important aquaculture species.
KW - 5S rDNA
KW - Evolutionary relationship
KW - Mitochondrial genome
KW - Mitten crabs
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U2 - 10.1016/j.aaf.2017.10.004
DO - 10.1016/j.aaf.2017.10.004
M3 - Article
AN - SCOPUS:85066765795
SN - 2096-1758
VL - 2
SP - 256
EP - 261
JO - Aquaculture and Fisheries
JF - Aquaculture and Fisheries
IS - 6
ER -