EWS/FLI-1 fusion signal inserted into chromosome 11 in one patient with morphologic features of Ewing sarcoma, but lacking t(11;22)

Jacqueline R. Batanian; Julia A. Bridge; Robert Wickert; Carol Vogler; Bharti Gadre; Yufeng Huang

doi:10.1016/S0165-4608(01)00563-5

EWS/FLI-1 fusion signal inserted into chromosome 11 in one patient with morphologic features of Ewing sarcoma, but lacking t(11;22)

Jacqueline R. Batanian, Julia A. Bridge, Robert Wickert, Carol Vogler, Bharti Gadre, Yufeng Huang

Pathology & Microbiology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A reciprocal t(11;22)(q24;q12) is found in 85% of Ewing sarcomas (ES) cases. We report a case of a child with ES, in whom trisomy 8 was observed as the sole chromosomal abnormality. Fluorescence in situ hybridization - using a set of probes that localize to 22q12 (EWS) and 11q24 (FLI-1) and usually show the translocation as fusion (red-green) signal on der(22) - showed a fusion signal on der(11) suggesting an insertion as the mechanism that led to the EWS-FLI-1 gene rearrangement. Reverse transcriptase-polymerase chain reaction studies revealed the presence of two EWS/FLI1 fusion gene products.

Original language	English (US)
Pages (from-to)	72-75
Number of pages	4
Journal	Cancer genetics and cytogenetics
Volume	133
Issue number	1
DOIs	https://doi.org/10.1016/S0165-4608(01)00563-5
State	Published - 2002

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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@article{b37d8503792e4fe6a83fede0849a8ff7,

title = "EWS/FLI-1 fusion signal inserted into chromosome 11 in one patient with morphologic features of Ewing sarcoma, but lacking t(11;22)",

abstract = "A reciprocal t(11;22)(q24;q12) is found in 85% of Ewing sarcomas (ES) cases. We report a case of a child with ES, in whom trisomy 8 was observed as the sole chromosomal abnormality. Fluorescence in situ hybridization - using a set of probes that localize to 22q12 (EWS) and 11q24 (FLI-1) and usually show the translocation as fusion (red-green) signal on der(22) - showed a fusion signal on der(11) suggesting an insertion as the mechanism that led to the EWS-FLI-1 gene rearrangement. Reverse transcriptase-polymerase chain reaction studies revealed the presence of two EWS/FLI1 fusion gene products.",

author = "Batanian, {Jacqueline R.} and Bridge, {Julia A.} and Robert Wickert and Carol Vogler and Bharti Gadre and Yufeng Huang",

note = "Funding Information: The authors would like to thank Ms. Cookie Knott and Ms. Sophie Hauxwell for their technical assistance. This work was supported in part by grants from the John A. Wiebe Children's Health Care Fund and the Scott Carter Foundation.",

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doi = "10.1016/S0165-4608(01)00563-5",

language = "English (US)",

volume = "133",

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T1 - EWS/FLI-1 fusion signal inserted into chromosome 11 in one patient with morphologic features of Ewing sarcoma, but lacking t(11;22)

AU - Batanian, Jacqueline R.

AU - Bridge, Julia A.

AU - Wickert, Robert

AU - Vogler, Carol

AU - Gadre, Bharti

AU - Huang, Yufeng

N1 - Funding Information: The authors would like to thank Ms. Cookie Knott and Ms. Sophie Hauxwell for their technical assistance. This work was supported in part by grants from the John A. Wiebe Children's Health Care Fund and the Scott Carter Foundation.

PY - 2002

Y1 - 2002

N2 - A reciprocal t(11;22)(q24;q12) is found in 85% of Ewing sarcomas (ES) cases. We report a case of a child with ES, in whom trisomy 8 was observed as the sole chromosomal abnormality. Fluorescence in situ hybridization - using a set of probes that localize to 22q12 (EWS) and 11q24 (FLI-1) and usually show the translocation as fusion (red-green) signal on der(22) - showed a fusion signal on der(11) suggesting an insertion as the mechanism that led to the EWS-FLI-1 gene rearrangement. Reverse transcriptase-polymerase chain reaction studies revealed the presence of two EWS/FLI1 fusion gene products.

AB - A reciprocal t(11;22)(q24;q12) is found in 85% of Ewing sarcomas (ES) cases. We report a case of a child with ES, in whom trisomy 8 was observed as the sole chromosomal abnormality. Fluorescence in situ hybridization - using a set of probes that localize to 22q12 (EWS) and 11q24 (FLI-1) and usually show the translocation as fusion (red-green) signal on der(22) - showed a fusion signal on der(11) suggesting an insertion as the mechanism that led to the EWS-FLI-1 gene rearrangement. Reverse transcriptase-polymerase chain reaction studies revealed the presence of two EWS/FLI1 fusion gene products.

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SN - 0165-4608

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JF - Cancer genetics and cytogenetics

IS - 1

ER -

EWS/FLI-1 fusion signal inserted into chromosome 11 in one patient with morphologic features of Ewing sarcoma, but lacking t(11;22)

Abstract

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