Excretion of phenol red by the Necturus kidney

G. A. Tanner, P. K. Carmines, W. B. Kinter

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Phenol red (phenolsulfonphthalein, PSP) is thought to be secreted by proximal kidney tubules in all vertebrates. The present study examined PSP transport by the kidney of the salamander, Necturus maculosus. In Necturus kidneys perfused with oxygenated Ringer solution, the PSP/creatinine clearance ratio was unity. Perfusion with 1 mM octanoate converted net p-aminohippurate (PAH) reabsorption to net secretion, but had no effect on PSP. In seven urethan-anesthetized Necturi, the PSP/inulin clearance ratio averaged 0.85 ± 0.21 (SD), not significantly different from unity. Thin slices from Necturus kidneys incubated in vitro for 2 h failed to accumulate PSP; slice-to-medium (S/M) concentration ratios averaged 0.8 ± 0.2 (n=6). With frog kidney slices, (S/M)PSP was 9.6 ± 1.4 (n=6). Necturus kidney slices accumulated PAH ((S/M)PAH = 4.1 ± 0.7) (n=6), but uptake was not inhibited by 1 mM PSP. We conclude that Necturus kidney tubules transport PAH, but do not transport PSP. These results are consistent with the hypothesis that the organic acid secretory system in most animals involves several carriers.

Original languageEnglish (US)
Pages (from-to)F442-F447
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume5
Issue number5
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Physiology

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