Exercise Training Attenuates Upregulation of p47phox and p67phox in Hearts of Diabetic Rats

Neeru M. Sharma, Brandon Rabeler, Hong Zheng, Eugenia Raichlin, Kaushik P. Patel

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Exercise training (ExT) is currently being used as a nonpharmacological strategy to improve cardiac function in diabetic patients. However, the molecular mechanism(s) underlying its beneficial effects remains poorly understood. Oxidative stress is known to play a key role in the pathogenesis of diabetic cardiomyopathy and one of the enzyme systems that produce reactive oxygen species is NADH/NADPH oxidase. The goal of this study was to investigate the effect of streptozotocin- (STZ-) induced diabetes on expression of p 47 p h o x and p 67 p h o x, key regulatory subunits of NADPH oxidase, in cardiac tissues and determine whether ExT can attenuate these changes. Four weeks after STZ treatment, expression of p 47 p h o x and p 67 p h o x increased 2.3-fold and 1.6-fold, respectively, in left ventricles of diabetic rats and these increases were attenuated with three weeks of ExT, initiated 1 week after onset of diabetes. In atrial tissues, there was increased expression of p 47 p h o x (74%), which was decreased by ExT in diabetic rats. Furthermore, increased collagen III levels in diabetic hearts (52%) were significantly reduced by ExT. Taken together, ExT attenuates the increased expression of p 47 p h o x and p 67 p h o x in the hearts of diabetic rats which could be an underlying mechanism for improving intracardiac matrix and thus cardiac function and prevent cardiac remodeling in diabetic cardiomyopathy.

Original languageEnglish (US)
Article number5868913
JournalOxidative medicine and cellular longevity
StatePublished - 2016

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Cell Biology

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