Exercise training improves vascular mitochondrial function

Song Young Park, Matthew J. Rossman, Jayson R. Gifford, Leena P. Bharath, Johann Bauersachs, Russell S. Richardson, E. Dale Abel, J. David Symons, Christian Riehle

Research output: Contribution to journalReview articlepeer-review

37 Scopus citations

Abstract

Exercise training is recognized to improve cardiac and skeletal muscle mitochondrial respiratory capacity; however, the impact of chronic exercise on vascular mitochondrial respiratory function is unknown. We hypothesized that exercise training concomitantly increases both vascular mitochondrial respiratory capacity and vascular function. Arteries from both sedentary (SED) and swim-trained (EX, 5 wk) mice were compared in terms of mitochondrial respiratory function, mitochondrial content, markers of mitochondrial biogenesis, redox balance, nitric oxide (NO) signaling, and vessel function. Mitochondrial complex I and complex I + II state 3 respiration and the respiratory control ratio (complex I + II state 3 respiration/complex I state 2 respiration) were greater in vessels from EX relative to SED mice, despite similar levels of arterial citrate synthase activity and mitochondrial DNA content. Furthermore, compared with the SED mice, arteries from EX mice displayed elevated transcript levels of peroxisome proliferative activated receptor-γ coactivator-1 α and the down stream target scytochrome c oxidase subunit IV isoform 1, isocitrate dehydrogenase (Idh) 2, and Idh3a, increased manganese superoxide dismutase protein expression, increased endothelial NO synthase phosphorylation (Ser1177), and suppressed reactive oxygen species generation (all P < 0.05). Although there were no differences in EX and SED mice concerning endothelium-dependent and endothelium-independent vasorelaxation, phenylephrine-induced vasocontraction was blunted in vessels from EX compared with SED mice, and this effect was normalized by NOS inhibition. These training-induced increases in vascular mitochondrial respiratory capacity and evidence of improved redox balance, which may, at least in part, be attributable to elevated NO bioavailability, have the potential to protect against ageand disease-related challenges to arterial function.

Original languageEnglish (US)
Pages (from-to)H821-H829
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume310
Issue number7
DOIs
StatePublished - Apr 2016
Externally publishedYes

Keywords

  • Arterial function
  • Mitochondria
  • Redox balance
  • Vasculature

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Exercise training improves vascular mitochondrial function'. Together they form a unique fingerprint.

Cite this