Expanding the mouse embryonic stem cell proteome: Combining three proteomic approaches

Rebekah L. Gundry, Irina Tchernyshyov, Shijun Sheng, Yelena Tarasova, Kimberly Raginski, Kenneth R. Boheler, Jennifer E. Van Eyk

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins.

Original languageEnglish (US)
Pages (from-to)2728-2732
Number of pages5
JournalProteomics
Volume10
Issue number14
DOIs
StatePublished - Jul 1 2010

Keywords

  • 1-D gel electrophoresis
  • 2-D chromatography
  • Cell biology
  • Embryonic stem cell
  • Isoforms
  • Shotgun proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Gundry, R. L., Tchernyshyov, I., Sheng, S., Tarasova, Y., Raginski, K., Boheler, K. R., & Van Eyk, J. E. (2010). Expanding the mouse embryonic stem cell proteome: Combining three proteomic approaches. Proteomics, 10(14), 2728-2732. https://doi.org/10.1002/pmic.201000039