Expansion of two-dimension electrospun nanofiber mats into threedimension scaffolds

Emily Keit, Shixuan Chen, Hongjun Wang, Jingwei Xie

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Electrospinning has been the preferred technology in producing a synthetic, functional scaffold due to the biomimicry to extracellular matrix and the ease control of composition, structure, and diameter of fibers. However, despite these advantages, traditional electrospun nanofiber scaffolds come with limitations including disorganized nanofiber orientation, low porosity, small pore size, and mainly two-dimensional mats. As such, there is a great need for developing a new process for fabricating electrospun nanofiber scaffolds that can overcome the above limitations. Herein, a novel and simple method is outlined. A traditional 2D nanofiber mat is transformed into a 3D scaffold with desired thickness, gap distance, porosity, and nanotopographic cues to allow for cell seeding and proliferation through the depressurization of subcritical CO 2 fluid. In addition to providing a scaffold for tissue regeneration to occur, this method also provides the opportunity to encapsulate bioactive molecules such as antimicrobial peptides for local drug delivery. The CO 2 expanded nanofiber scaffolds hold great potential in tissue regeneration, wound healing, 3D tissue modeling, and topical drug delivery.

Original languageEnglish (US)
Article numbere58918
JournalJournal of Visualized Experiments
Issue number143
StatePublished - Jan 2019


  • Antimicrobial peptides
  • Drug delivery
  • Electrospun nanofibers
  • Expand
  • Genetics
  • Issue 143
  • Subcritical CO
  • Three-dimension scaffolds
  • Tissue regeneration
  • Two-dimension mats

ASJC Scopus subject areas

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


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