TY - JOUR
T1 - Expansion of two-dimension electrospun nanofiber mats into threedimension scaffolds
AU - Keit, Emily
AU - Chen, Shixuan
AU - Wang, Hongjun
AU - Xie, Jingwei
N1 - Funding Information:
This work was supported by grants from the National Institute of General Medical Science (NIGMS) at the NIH (2P20 GM103480-06 and 1R01GM123081 to J.X.), the Otis Glebe Medical Research Foundation, NE LB606, and startup funds from the University of Nebraska Medical Center.
Publisher Copyright:
© 2019 Journal of Visualized Experiments.
PY - 2019/1
Y1 - 2019/1
N2 - Electrospinning has been the preferred technology in producing a synthetic, functional scaffold due to the biomimicry to extracellular matrix and the ease control of composition, structure, and diameter of fibers. However, despite these advantages, traditional electrospun nanofiber scaffolds come with limitations including disorganized nanofiber orientation, low porosity, small pore size, and mainly two-dimensional mats. As such, there is a great need for developing a new process for fabricating electrospun nanofiber scaffolds that can overcome the above limitations. Herein, a novel and simple method is outlined. A traditional 2D nanofiber mat is transformed into a 3D scaffold with desired thickness, gap distance, porosity, and nanotopographic cues to allow for cell seeding and proliferation through the depressurization of subcritical CO 2 fluid. In addition to providing a scaffold for tissue regeneration to occur, this method also provides the opportunity to encapsulate bioactive molecules such as antimicrobial peptides for local drug delivery. The CO 2 expanded nanofiber scaffolds hold great potential in tissue regeneration, wound healing, 3D tissue modeling, and topical drug delivery.
AB - Electrospinning has been the preferred technology in producing a synthetic, functional scaffold due to the biomimicry to extracellular matrix and the ease control of composition, structure, and diameter of fibers. However, despite these advantages, traditional electrospun nanofiber scaffolds come with limitations including disorganized nanofiber orientation, low porosity, small pore size, and mainly two-dimensional mats. As such, there is a great need for developing a new process for fabricating electrospun nanofiber scaffolds that can overcome the above limitations. Herein, a novel and simple method is outlined. A traditional 2D nanofiber mat is transformed into a 3D scaffold with desired thickness, gap distance, porosity, and nanotopographic cues to allow for cell seeding and proliferation through the depressurization of subcritical CO 2 fluid. In addition to providing a scaffold for tissue regeneration to occur, this method also provides the opportunity to encapsulate bioactive molecules such as antimicrobial peptides for local drug delivery. The CO 2 expanded nanofiber scaffolds hold great potential in tissue regeneration, wound healing, 3D tissue modeling, and topical drug delivery.
KW - Antimicrobial peptides
KW - Drug delivery
KW - Electrospun nanofibers
KW - Expand
KW - Genetics
KW - Issue 143
KW - Subcritical CO
KW - Three-dimension scaffolds
KW - Tissue regeneration
KW - Two-dimension mats
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U2 - 10.3791/58918
DO - 10.3791/58918
M3 - Article
C2 - 30663697
AN - SCOPUS:85060150484
VL - 2019
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
IS - 143
M1 - e58918
ER -