Experience with routine vaginal pH testing in a family practice setting

Adriana J. Pavletic, Stephen E. Hawes, Jenenne A. Geske, Kathy Bringe, Susan H. Polack

Research output: Contribution to journalReview articlepeer-review

27 Scopus citations


Background: Despite recommendations by Centers for Disease Control and the American College of Obstetricians and Gynecologists, pH testing is infrequently performed during the evaluation of vaginitis. Consequently, little information exists on its use in a primary care setting. Objective: The aim of this study was to describe our experience with routine pH testing, particularly the relationship between symptoms, pH and wet-mount microscopy. Method: A retrospective chart review was performed on 203 consecutive cases evaluated for vaginitis by wet-mount microscopy. Results: Of the 203 cases, 21 had normal pH and no symptoms and 182 had symptoms, elevated pH or both; 85% of cases had abnormal wet-mount findings, including 75% with clue cells, 14% with Trichomonas vaginalis, 13% with yeast and 14% with mixed infections. Asymptomatic infection was present in 42% of cases with clue cells alone, 44% of cases with Trichomonas vaginalis alone, 38% of all trichomoniasis cases and 33% of cases with mixed infections. Elevated pH was associated with clue cells (p < 0.001), trichomoniasis (p = 0.01) and mixed infections (p = 0.003). Normal pH was associated with negative wet mount (p < 0.001) and to a lesser degree with uncomplicated vulvovaginal candidiasis (p = 0.06). Conclusion: Routine pH testing increased detection of trichomoniasis and bacterial vaginosis by prompting microscopy in a significant proportion of asymptomatic cases.

Original languageEnglish (US)
Pages (from-to)63-68
Number of pages6
JournalInfectious Disease in Obstetrics and Gynecology
Issue number2
StatePublished - Jun 2004


  • Asymptomatic vaginitis
  • Bacterial vaginosis
  • Trichomonas vaginalis
  • Vaginal pH

ASJC Scopus subject areas

  • Dermatology
  • Obstetrics and Gynecology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Experience with routine vaginal pH testing in a family practice setting'. Together they form a unique fingerprint.

Cite this