@article{a8bdfe83d8e94ed9af0f8a91624fa04d,
title = "Experimental cryptorchidism enhances testicular susceptibility to dibutyl phthalate or acrylamide in Sprague-Dawley rats",
abstract = "Cryptorchidism (CPT), the most common male congenital abnormality, is variably associated with other male reproductive tract problems. We evaluated if cryptorchid rats develop enhanced testicular susceptibility to dibutyl phthalate (DBP) or acrylamide (AA) after extended exposure. Three studies with rats were performed: (1) in utero and postnatal exposure to DBP or AA; (2) establishment of CPT and orchiopexy; and (3) in utero and postnatal exposures to DBP or AA associated with CPT/orchiopexy. Seminiferous tubules were histologically scored according to the severity of lesions: (1) Rats exposed to DBP (score 1.5) or AA (score 1.1) presented mostly preserved spermatogenesis. Some seminiferous tubules showed vacuolated germinative epithelium, germ cell apoptosis, and a Sertoli cell-only (SCO) pattern. (2) CPT (score 3.3) resulted in decreased absolute testes weights, degenerated and SCO tubules, and spermatogenesis arrest that were reversed by orchiopexy (score 1.1). (3) Exposure to DBP or AA with CPT/orchiopexy led to atrophic testes, spermatogenesis arrest, germ cell exfoliation/multinucleation, and SCO tubules (both chemicals score 2.5). Exposure to chemicals such as DBP or AA prevented the recovery of cryptorchid testes by orchiopexy. The possible role of environmental contaminants should be considered when looking for factors that modulate human testicular disorders associated with CPT.",
keywords = "Cryptorchidism, acrylamide, dibutyl phthalate, histology, testicular germ cells",
author = "{de Souza}, {N. P.} and Cardoso, {AP Ferragut} and Gomide, {L. M.M.} and Lima, {T. R.R.} and Miot, {H. A.} and Martino-Andrade, {A. J.} and Arnold, {L. L.} and Pennington, {K. L.} and Cohen, {S. M.} and {de Camargo}, {J. L.V.} and {Nascimento e Pontes}, {M. G.}",
note = "Funding Information: The authors are grateful to Carlos M?rcio N?brega de Jesus, Botucatu Medical School, UNESP?Univ Estadual Paulista, Botucatu Campus, SP, Brazil, for assistance with the surgical procedures; Paulo Roberto Cardoso and Paulo Cesar Georgete for helpful technical assistance; Fred and Pamela Buffett Cancer Center Tissue Sciences Facility Shared Resource, supported by the National Cancer Institute under award number P30 CA036727, Center for Evaluation of Environmental Impact of Human Health (TOXICAM) and S?o Paulo State University (UNESP). The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (FAPESP) [Grant No. 2012/09873-4]; Conselho Nacional de Pesquisa (CNPq) [Grant 132667/2013-4]. Funding Information: The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the Fundac¸{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo (FAPESP) [Grant No. 2012/09873-4]; Conselho Nacional de Pesquisa (CNPq) [Grant 132667/2013-4]. Funding Information: The authors are grateful to Carlos M{\'a}rcio N{\'o}brega de Jesus, Botucatu Medical School, UNESP—Univ Estadual Paulista, Botucatu Campus, SP, Brazil, for assistance with the surgical procedures; Paulo Roberto Cardoso and Paulo Cesar Georgete for helpful technical assistance; Fred and Pamela Buffett Cancer Center Tissue Sciences Facility Shared Resource, supported by the National Cancer Institute under award number P30 CA036727, Center for Evaluation of Environmental Impact of Human Health (TOXICAM) and S{\~a}o Paulo State University (UNESP). Publisher Copyright: {\textcopyright} The Author(s) 2019.",
year = "2019",
month = aug,
day = "1",
doi = "10.1177/0960327119845040",
language = "English (US)",
volume = "38",
pages = "899--913",
journal = "Human and Experimental Toxicology",
issn = "0960-3271",
publisher = "SAGE Publications Inc.",
number = "8",
}