Experimental measurement of bioaerosol concentrations and containment in long-term care environments

Ehsan Mousavi, Kevin Grosskopf, Phil Arnold, Roger Lautz, Josephine Lau

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Although many long-term care (LTC) facilities have implemented measures to isolate infectious residents from the general population, most are not designed for airborne infection control, and guidance for retrofitting existing LTC spaces for airborne isolation is limited. The purpose of this study was to evaluate the effect of ventilation, negative pressure, airflow barriers, and other retrofit measures on bioaerosol concentration and movement within long-term care LTC environments. To that end, a series of bioaerosol measurements was performed in an LTC facility under various pressurization and airflow configurations. We arranged active air sampling of DNA-tagged solutions release in the LTC environment, followed by quantitative polymerase chain reaction (qPCR) techniques to measure the released DNA in various spatial locations. Results from aerosol testing in an actual LTC facility suggest that increasing both total and outside ventilation rate had a modest and disproportional effect on the containment of bioaerosols, yet it significantly reduced the time necessary to remove 99% of aerosols from 3 h to approximately 40 min. Significant reductions in aerosol mobility between resident rooms, corridors, and common spaces were also observed with respect to negative room pressurization and anterooms.

Original languageEnglish (US)
Article number109415
JournalBuilding and Environment
StatePublished - Sep 2022


  • Air quality
  • Bioaerosol measurements
  • Built environment
  • Disease transmission
  • Long term care

ASJC Scopus subject areas

  • Environmental Engineering
  • Civil and Structural Engineering
  • Geography, Planning and Development
  • Building and Construction


Dive into the research topics of 'Experimental measurement of bioaerosol concentrations and containment in long-term care environments'. Together they form a unique fingerprint.

Cite this