Experimental Retinal Detachment: VIII. Retinochoroidal Horseradish Peroxidase Diffusion Across the Blood-Retinal Barrier

Carol B. Toris, Jonathan E. Pederson

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Unilateral rhegmatogenous retinal detachments in 13 cynomolgus monkeys were studied with horseradish peroxidase (HRP). When injected subretinally in six eyes, HRP did not diffuse anteriorly into the sensory retina and penetrated posteriorly through the zonulae occludentes of the retinal pigment epithelium (RPE) in only two eyes. In seven eyes, tracer was detected after intravitreal HRP injection throughout the sensory retina, the basal lamina of retinal blood vessels, and the subretinal space, but did not penetrate through the RPE. In 13 control eyes (with vitrectomy), intravitreal HRP penetrated the sensory retina and the basal lamina surrounding inner retinal blood vessels. These results confirm that the zonulae occludentes of the RPE and retinal blood vessels remain intact in most eyes after rhegmatogenous retinal detachment. Furthermore, the HRP staining patterns suggest a posteriorly directed movement of fluid across the RPE and possible fluid absorption by retinal blood vessels.

Original languageEnglish (US)
Pages (from-to)266-269
Number of pages4
JournalArchives of Ophthalmology
Volume103
Issue number2
DOIs
StatePublished - Feb 1985

ASJC Scopus subject areas

  • Ophthalmology

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