Exploratory study on the effects of biodegradable nanoparticles with drugs on malignant b cells and on a human/mouse model of burkitt lymphoma

Gustavo H. Marín; Eduardo Mansilla; Nelly Mezzaroba; Sonia Zorzet; Luis Núñez; Gustavo Larsen; José M. Tau; Alberto Maceira; Ruben Spret; Carol Mertz; Sabrina Ingrao; Claudio Tripodo; Francesco Tedesco; Paolo Macor

doi:10.2174/157488410793352058

Exploratory study on the effects of biodegradable nanoparticles with drugs on malignant b cells and on a human/mouse model of burkitt lymphoma

Gustavo H. Marín, Eduardo Mansilla, Nelly Mezzaroba, Sonia Zorzet, Luis Núñez, Gustavo Larsen, José M. Tau, Alberto Maceira, Ruben Spret, Carol Mertz, Sabrina Ingrao, Claudio Tripodo, Francesco Tedesco, Paolo Macor

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The aim of this study was to determine if Rituximab coated Biodegradable Nanoparticles (BNPs) loaded with Chlorambucil and Hydroxychloroquine could induce apoptosis of B-Chronic Lymphocytic Leukemia (B-CLL), MEC-1 and BJAB cells in vitro and evaluate their toxic and therapeutic effects on a Human/Mouse Model of Burkitt Lymphoma at an exploratory, proof of concept scale. We found that Rituximab-Chlorambucil-Hydroxychloroquine BNPs induce a decrease in cell viability of malignant B cells in a dose-dependent manner. The mediated cytotoxicity resulted from apoptosis, and was confirmed by monitoring the B-CLL cells after Annexin V/propidium iodide staining. Additional data revealed that these BNPs were non toxic for healthy animals, and had prolonged survival in this mice model of human lymphoma.

Original language	English (US)
Pages (from-to)	246-250
Number of pages	5
Journal	Current Clinical Pharmacology
Volume	5
Issue number	4
DOIs	https://doi.org/10.2174/157488410793352058
State	Published - 2010
Externally published	Yes

Keywords

B cells
CLL
Chlorambucil
Hydroxychloroquine
Lymphoma
Nanoparticles
Rituximab

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
Pharmacology (medical)

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10.2174/157488410793352058

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Marín, G. H., Mansilla, E., Mezzaroba, N., Zorzet, S., Núñez, L., Larsen, G., Tau, J. M., Maceira, A., Spret, R., Mertz, C., Ingrao, S., Tripodo, C., Tedesco, F., & Macor, P. (2010). Exploratory study on the effects of biodegradable nanoparticles with drugs on malignant b cells and on a human/mouse model of burkitt lymphoma. Current Clinical Pharmacology, 5(4), 246-250. https://doi.org/10.2174/157488410793352058

Marín, GH, Mansilla, E, Mezzaroba, N, Zorzet, S, Núñez, L, Larsen, G, Tau, JM, Maceira, A, Spret, R, Mertz, C, Ingrao, S, Tripodo, C, Tedesco, F & Macor, P 2010, 'Exploratory study on the effects of biodegradable nanoparticles with drugs on malignant b cells and on a human/mouse model of burkitt lymphoma', Current Clinical Pharmacology, vol. 5, no. 4, pp. 246-250. https://doi.org/10.2174/157488410793352058

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abstract = "The aim of this study was to determine if Rituximab coated Biodegradable Nanoparticles (BNPs) loaded with Chlorambucil and Hydroxychloroquine could induce apoptosis of B-Chronic Lymphocytic Leukemia (B-CLL), MEC-1 and BJAB cells in vitro and evaluate their toxic and therapeutic effects on a Human/Mouse Model of Burkitt Lymphoma at an exploratory, proof of concept scale. We found that Rituximab-Chlorambucil-Hydroxychloroquine BNPs induce a decrease in cell viability of malignant B cells in a dose-dependent manner. The mediated cytotoxicity resulted from apoptosis, and was confirmed by monitoring the B-CLL cells after Annexin V/propidium iodide staining. Additional data revealed that these BNPs were non toxic for healthy animals, and had prolonged survival in this mice model of human lymphoma.",

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AU - Núñez, Luis

AU - Larsen, Gustavo

AU - Tau, José M.

AU - Maceira, Alberto

AU - Spret, Ruben

AU - Mertz, Carol

AU - Ingrao, Sabrina

AU - Tripodo, Claudio

AU - Tedesco, Francesco

AU - Macor, Paolo

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AB - The aim of this study was to determine if Rituximab coated Biodegradable Nanoparticles (BNPs) loaded with Chlorambucil and Hydroxychloroquine could induce apoptosis of B-Chronic Lymphocytic Leukemia (B-CLL), MEC-1 and BJAB cells in vitro and evaluate their toxic and therapeutic effects on a Human/Mouse Model of Burkitt Lymphoma at an exploratory, proof of concept scale. We found that Rituximab-Chlorambucil-Hydroxychloroquine BNPs induce a decrease in cell viability of malignant B cells in a dose-dependent manner. The mediated cytotoxicity resulted from apoptosis, and was confirmed by monitoring the B-CLL cells after Annexin V/propidium iodide staining. Additional data revealed that these BNPs were non toxic for healthy animals, and had prolonged survival in this mice model of human lymphoma.

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Exploratory study on the effects of biodegradable nanoparticles with drugs on malignant b cells and on a human/mouse model of burkitt lymphoma

Abstract

Keywords

ASJC Scopus subject areas

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