Exploratory study on the effects of biodegradable nanoparticles with drugs on malignant b cells and on a human/mouse model of burkitt lymphoma

Gustavo H. Marín, Eduardo Mansilla, Nelly Mezzaroba, Sonia Zorzet, Luis Núñez, Gustavo Larsen, José M. Tau, Alberto Maceira, Ruben Spret, Carol Mertz, Sabrina Ingrao, Claudio Tripodo, Francesco Tedesco, Paolo Macor

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The aim of this study was to determine if Rituximab coated Biodegradable Nanoparticles (BNPs) loaded with Chlorambucil and Hydroxychloroquine could induce apoptosis of B-Chronic Lymphocytic Leukemia (B-CLL), MEC-1 and BJAB cells in vitro and evaluate their toxic and therapeutic effects on a Human/Mouse Model of Burkitt Lymphoma at an exploratory, proof of concept scale. We found that Rituximab-Chlorambucil-Hydroxychloroquine BNPs induce a decrease in cell viability of malignant B cells in a dose-dependent manner. The mediated cytotoxicity resulted from apoptosis, and was confirmed by monitoring the B-CLL cells after Annexin V/propidium iodide staining. Additional data revealed that these BNPs were non toxic for healthy animals, and had prolonged survival in this mice model of human lymphoma.

Original languageEnglish (US)
Pages (from-to)246-250
Number of pages5
JournalCurrent Clinical Pharmacology
Volume5
Issue number4
DOIs
StatePublished - 2010

Keywords

  • B cells
  • CLL
  • Chlorambucil
  • Hydroxychloroquine
  • Lymphoma
  • Nanoparticles
  • Rituximab

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

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    Marín, G. H., Mansilla, E., Mezzaroba, N., Zorzet, S., Núñez, L., Larsen, G., Tau, J. M., Maceira, A., Spret, R., Mertz, C., Ingrao, S., Tripodo, C., Tedesco, F., & Macor, P. (2010). Exploratory study on the effects of biodegradable nanoparticles with drugs on malignant b cells and on a human/mouse model of burkitt lymphoma. Current Clinical Pharmacology, 5(4), 246-250. https://doi.org/10.2174/157488410793352058