TY - JOUR
T1 - Expression and Down‐Regulation by Retinoic Acid of IGF Binding Protein‐2 and ‐4 in Medium from Human Neuroblastoma Cells
AU - Bernardini, Sergio
AU - Cianfarani, Stefano
AU - Spagnoli, Anna
AU - Annicchiarico‐Petruzzelli, Margherita
AU - Melino, Gerry
AU - Massoud, Renato
AU - Boscherini, Brunetto
AU - Finazzi‐Agró, Alessandro
AU - Rosenfeld, Ron G.
AU - Federici, Giorgio
PY - 1994/8
Y1 - 1994/8
N2 - Insulin‐like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF‐I, IGF‐II and truncated Des(1‐3)IGF‐l in conditioned medium (CM) of the human neuroblastoma SK‐N‐BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37 kDa and 25 kDa. Following immunoprecipitation, they turned out to be IGFBP‐2 and ‐4, respectively. The RA‐induced differentiation in SK‐N‐BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48 h (32% and 24% of control, respectively) and 72 h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF‐I, which induced a marked decrease in both the 37 kDa and 25kDa bands after 72 h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA‐induced differentiation in human neuroblastoma cells.
AB - Insulin‐like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF‐I, IGF‐II and truncated Des(1‐3)IGF‐l in conditioned medium (CM) of the human neuroblastoma SK‐N‐BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37 kDa and 25 kDa. Following immunoprecipitation, they turned out to be IGFBP‐2 and ‐4, respectively. The RA‐induced differentiation in SK‐N‐BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48 h (32% and 24% of control, respectively) and 72 h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF‐I, which induced a marked decrease in both the 37 kDa and 25kDa bands after 72 h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA‐induced differentiation in human neuroblastoma cells.
KW - insulin‐like growth factors
KW - insulin‐like growth factor‐binding proteins
KW - neuroblastoma
KW - retinoic acid
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U2 - 10.1111/j.1365-2826.1994.tb00601.x
DO - 10.1111/j.1365-2826.1994.tb00601.x
M3 - Article
C2 - 7527270
AN - SCOPUS:0028168651
SN - 0953-8194
VL - 6
SP - 409
EP - 413
JO - Journal of Neuroendocrinology
JF - Journal of Neuroendocrinology
IS - 4
ER -