TY - JOUR
T1 - Expression and localization of carbonic anhydrase in bovine mammary gland and secretion in milk
AU - Kitade, Keitaro
AU - Nishita, Toshiho
AU - Yamato, Mitsugu
AU - Sakamoto, Kazuhito
AU - Hagino, Akihiko
AU - Katoh, Kazuo
AU - Obara, Yoshiaki
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Little attention has been paid to carbonic anhydrase VI (CA VI), a secretory type isozyme, in the bovine mammary gland, although the gland is an important exocrine gland and CA VI is known to localize in exocrine glands such as salivary and lacrimal glands in various animal species. In the present study mRNA expression and protein localization of CA VI in isolated gland tissues and in cloned epithelial cells from the mammary gland of Holstein cows (Bos taurus) were observed by reverse transcript polymerase chain reaction and immunocytochemistry. Also, changes of CA VI concentrations in milk were measured for 2 months postpartum by an enzyme-linked immunosorbent assay. CA VI gene expression was detected in the gland tissues and epithelial cells, and CA VI protein was localized in the cytoplasm of the epithelial cells. Colostrum contained the highest concentration of CA VI protein (100 ng/ml), decreasing in an exponential manner (P<0.001). We conclude that bovine mammary epithelial cells synthesize and secrete CA VI in colostrum at higher concentration than in normal milk, implying its role to compensate for low CA VI secretion in neonatal calves.
AB - Little attention has been paid to carbonic anhydrase VI (CA VI), a secretory type isozyme, in the bovine mammary gland, although the gland is an important exocrine gland and CA VI is known to localize in exocrine glands such as salivary and lacrimal glands in various animal species. In the present study mRNA expression and protein localization of CA VI in isolated gland tissues and in cloned epithelial cells from the mammary gland of Holstein cows (Bos taurus) were observed by reverse transcript polymerase chain reaction and immunocytochemistry. Also, changes of CA VI concentrations in milk were measured for 2 months postpartum by an enzyme-linked immunosorbent assay. CA VI gene expression was detected in the gland tissues and epithelial cells, and CA VI protein was localized in the cytoplasm of the epithelial cells. Colostrum contained the highest concentration of CA VI protein (100 ng/ml), decreasing in an exponential manner (P<0.001). We conclude that bovine mammary epithelial cells synthesize and secrete CA VI in colostrum at higher concentration than in normal milk, implying its role to compensate for low CA VI secretion in neonatal calves.
KW - Carbonic anhydrase VI
KW - Colostrum
KW - Cow
KW - Immunocytochemistry
KW - Mammary epithelial cells
KW - Neonate
KW - RT-PCR
KW - Secretion
UR - http://www.scopus.com/inward/record.url?scp=0037304284&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037304284&partnerID=8YFLogxK
U2 - 10.1016/S1095-6433(02)00268-4
DO - 10.1016/S1095-6433(02)00268-4
M3 - Article
C2 - 12547264
AN - SCOPUS:0037304284
SN - 1095-6433
VL - 134
SP - 349
EP - 354
JO - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
JF - Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
IS - 2
ER -