Abstract
While characterizing various splice forms of p120 catenin, we observed what appeared to be a novel posttranslational modification of p120, resulting in a higher molecular weight form that was dependent on the splicing pattern. Further investigation revealed the higher molecular weight form to be a fusion protein between sequences encoded by the retroviral vector and p120. We found that the publicly available sequence of the vector we used does not agree with the experimental sequence. We caution other investigators to be aware of this potential artifact.
Original language | English (US) |
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Pages (from-to) | 49-53 |
Number of pages | 5 |
Journal | Analytical Biochemistry |
Volume | 395 |
Issue number | 1 |
DOIs | |
State | Published - Dec 1 2009 |
Keywords
- LZRS
- Retroviral vector
- p120 Catenin
- pBMN
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology