Expression of fibroblast growth factor receptors by embryonal carcinoma cells and early mouse embryos

W. J. Campbell, K. A. Miller, T. M. Anderson, J. D. Shull, A. Rizzino

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

We have previously shown that differentiation of embryonal carcinoma (EC) cells leads to both increased binding of FGF (fibroblast growth factor) and suppression of k-FGF expression. In the current study, we examined the expression of FGF receptors by EC cells, EC-derived differentiated cells and early mammalian embryos using the technique of reverse transcription- polymerase chain reaction (RT-PCR). We determined that both mouse, F9, and human, NT2/D1, EC cells as well as their differentiated counterparts express transcripts for two forms of FGF receptors, bek (bacterially expressed kinase) and flg (fms-like gene). In addition, we determined that mouse blastocysts express flg transcripts. The presence of FGF receptor transcripts in early embryos and the previous finding of FGF-related activity in medium conditioned by mouse blastocysts argue that the FGF family plays important roles during early mammalian development.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume28 A
Issue number1
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Expression of fibroblast growth factor receptors by embryonal carcinoma cells and early mouse embryos'. Together they form a unique fingerprint.

  • Cite this