Expression of fibroblast growth factor receptors by embryonal carcinoma cells and early mouse embryos

W. J. Campbell, K. A. Miller, T. M. Anderson, J. D. Shull, A. Rizzino

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We have previously shown that differentiation of embryonal carcinoma (EC) cells leads to both increased binding of FGF (fibroblast growth factor) and suppression of k-FGF expression. In the current study, we examined the expression of FGF receptors by EC cells, EC-derived differentiated cells and early mammalian embryos using the technique of reverse transcription- polymerase chain reaction (RT-PCR). We determined that both mouse, F9, and human, NT2/D1, EC cells as well as their differentiated counterparts express transcripts for two forms of FGF receptors, bek (bacterially expressed kinase) and flg (fms-like gene). In addition, we determined that mouse blastocysts express flg transcripts. The presence of FGF receptor transcripts in early embryos and the previous finding of FGF-related activity in medium conditioned by mouse blastocysts argue that the FGF family plays important roles during early mammalian development.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume28 A
Issue number1
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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