Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice

Rakesh K. Singh, Mordechai Gutman, Robert Radinsky, Corazon D. Bucana, Isaiah J. Fidler

Research output: Contribution to journalArticle

332 Scopus citations

Abstract

We correlated the steady state transcription and protein secretion of interleukin 8 (IL-8) in 13 different human melanoma cell lines with their ability to grow and produce metastasis in nude mice. Highly metastatic cells expressed higher steady state levels of IL-8 mRNA transcripts than did low metastatic cells. In situ mRNA hybridization analyses confirmed the pattern of mRNA expression on a cellular level. Increased mRNA expression directly correlated with secretion of IL-8 protein as determined by enzyme-linked immunosorbent assay. Recombinant IL-8 stimulated the proliferation of low metastatic A375P cells in a dose-dependent manner, a stimulation that was abrogated by the use of a polyclonal antibody against IL-8. The data suggest that IL-8 can be an autocrine growth factor for human melanoma cells and that IL-8 is involved in melanoma metastasis.

Original languageEnglish (US)
Pages (from-to)3242-3247
Number of pages6
JournalCancer Research
Volume54
Issue number12
StatePublished - Jun 15 1994

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Singh, R. K., Gutman, M., Radinsky, R., Bucana, C. D., & Fidler, I. J. (1994). Expression of Interleukin 8 Correlates with the Metastatic Potential of Human Melanoma Cells in Nude Mice. Cancer Research, 54(12), 3242-3247.