Expression of oxidative stress-responsive genes and cytokine genes during caerulein-induced acute pancreatitis

F. U. Kai, Michael P. Sarras, Robert C. De Lisle, Glen K. Andrews

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


Oxidative stress and the inflammatory response may play roles in the pathogenesis of acute pancreatitis. Herein, we characterized pancreatic expression of oxidative stress-responsive gene [c-fos, heme oxygenase-1 (HO- 1), and metallothionein-I (MT-I) and cytokine genes [interleukin-1β (IL- 1β), IL-6, and tumor necrosis factor-α (TNF-α)] during caerulein-induced acute pancreatitis in the mouse. c-fos, HO-1 and MT-I mRNAs were coordinately and rapidly (3-7 h) upregulated, and HO-1 and MT-I protein levels were increased slightly in the pancreas during acute pancreatitis. In addition, IL-1β, IL-6, AND TNF-α mRNAs were rapidly (7 h) upregulated in the pancreas, and intrapancreatic IL-1β and IL-6 protein levels rapidly increased (3-fold and 6.4-fold, respectively) during acute pancreatitis. These studies suggest that oxidative stress and inflammation each occur in the pancreas during the early stages of acute pancreatitis. However, under a limited set of experimental conditions, we found that an insult that causes pancreatic oxidative stress (diethylmaleate) or one that induces an inflammatory response (bacterial lipopolysaccharide), or a combination of these agents, did not cause the changes characteristic of acute pancreatitis. Therefore, simply inducing oxidative stress and/or inflammation may be insufficient to initiate acute pancreatitis.

Original languageEnglish (US)
Pages (from-to)G696-G705
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number3 36-3
StatePublished - Sep 1997


  • C-fos
  • Glutathione
  • Heme oxygenase
  • Inflammation
  • Interleukin
  • Metallothionein
  • Mouse

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)


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