TY - JOUR
T1 - Expression of the major basement membrane-associated proteins during postnatal development in the murine cochlea
AU - Cosgrove, Dominic
AU - Rodgers, Kathryn D.
N1 - Funding Information:
Our sincere thanks to John (Skip) Kennedy for his help in the preparation of data for publication. Our thanks to Caroline Miller for the preparation of plastic embedded tissue sections. Thanks also to Daniel Mee han for setting up timed matings, and for the daily care and maintenance of animals. This work was supported by NIH Grant POl DC01813 from the NIDCD.
PY - 1997/3
Y1 - 1997/3
N2 - The major basement membrane-associated proteins, including laminin-1, fibronectin, heparan sulfate proteoglycan (HSP), and entactin, were examined by immunofluorescence microscopy during postnatal development of the mouse cochlea. Samples were collected every 2 days through 8 days, and again at 14 days after birth. In the neonate, staining for HSP entactin and laminin-l was barely visible; however, antibodies against fibronectin displayed intense immunoreactivity in nearly every cochlear tissue. Fibronectin is progressively inactivated in all tissues except the basilar membrane where it persists at high levels to adulthood. Laminin-1, entactin, and HSP illustrate remarkable temporal and spatial coordinate regulation. Elevated expression of these proteins is observed at 2 postnatal days (PND), and persists in the membranes surrounding the spiral ganglion cell bodies. Transient expression of laminin-1 and entactin, and to a lesser extent HSP, is observed from PND4 to PND8 in a track of membrane running from the interdental cells of the spiral limbus down the inner sulcus, across the basilar membrane, up the external sulcus to the spiral prominence, and branching into the spiral ligament ensheathing the root cell processes. By PND14 the abundance of these proteins is greatly reduced along this track. The abundance and dynamic regulation of these major basement membrane-associated proteins suggests that they playan important role in postnatal cochlear development.
AB - The major basement membrane-associated proteins, including laminin-1, fibronectin, heparan sulfate proteoglycan (HSP), and entactin, were examined by immunofluorescence microscopy during postnatal development of the mouse cochlea. Samples were collected every 2 days through 8 days, and again at 14 days after birth. In the neonate, staining for HSP entactin and laminin-l was barely visible; however, antibodies against fibronectin displayed intense immunoreactivity in nearly every cochlear tissue. Fibronectin is progressively inactivated in all tissues except the basilar membrane where it persists at high levels to adulthood. Laminin-1, entactin, and HSP illustrate remarkable temporal and spatial coordinate regulation. Elevated expression of these proteins is observed at 2 postnatal days (PND), and persists in the membranes surrounding the spiral ganglion cell bodies. Transient expression of laminin-1 and entactin, and to a lesser extent HSP, is observed from PND4 to PND8 in a track of membrane running from the interdental cells of the spiral limbus down the inner sulcus, across the basilar membrane, up the external sulcus to the spiral prominence, and branching into the spiral ligament ensheathing the root cell processes. By PND14 the abundance of these proteins is greatly reduced along this track. The abundance and dynamic regulation of these major basement membrane-associated proteins suggests that they playan important role in postnatal cochlear development.
KW - Basement membrane
KW - Cochlea
KW - Development
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U2 - 10.1016/S0378-5955(96)00203-1
DO - 10.1016/S0378-5955(96)00203-1
M3 - Article
C2 - 9083813
AN - SCOPUS:0031106746
SN - 0378-5955
VL - 105
SP - 159
EP - 170
JO - Hearing Research
JF - Hearing Research
IS - 1-2
ER -