Expression of three UDP-N-acetyl-α-D-galactosamine:polypeptide GalNAc N-Acetylagalactosaminyltransferases in adenocarcinoma cell lines

Marie E. Sutherlin, Isao Nishimori, Thomas Caffrey, Eric P. Bennett, Helle Hassan, Ulla Mandel, David Mack, Takeshi Iwamura, Henrik Clausen, Michael A. Hollingsworth

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56 Scopus citations

Abstract

The levels of mRNA expression of three UDP-N-acetyl-α-D- galactosamine:polypeptide GalNAc N-acetylgalactosaminyltransferases (GalNAc- transferases) were quantified for human adenocarcinoma cell lines from pancreas, colon, stomach, and breast. Two of the GalNAc-transferases, GalNAc- T1 and GalNAc-T2, were expressed constitutively and at low levels in most or all cell lines examined. A third GalNAc-transferase, GalNAc-T3, was differentially expressed. Well-differentiated adenocarcinoma cell lines expressed high levels and moderately differentiated cell lines expressed lower levels of GalNAc-T3. Cell lines classified as poorly differentiated failed to express GalNAc-T3 mRNA at levels that could be detected by Northern blot analysis. Differential expression of the GalNAc-T3 protein was confirmed in these cell lines by Western blotting. We propose that glycosylation in tumor cell lines may be regulated in part by differential expression of GalNAc-transferases, and we suggest that GalNAc-T3 gene expression may be a molecular indicator of differentiated adenocarcinoma.

Original languageEnglish (US)
Pages (from-to)4744-4748
Number of pages5
JournalCancer Research
Volume57
Issue number21
StatePublished - Nov 1 1997

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Sutherlin, M. E., Nishimori, I., Caffrey, T., Bennett, E. P., Hassan, H., Mandel, U., Mack, D., Iwamura, T., Clausen, H., & Hollingsworth, M. A. (1997). Expression of three UDP-N-acetyl-α-D-galactosamine:polypeptide GalNAc N-Acetylagalactosaminyltransferases in adenocarcinoma cell lines. Cancer Research, 57(21), 4744-4748.