Abstract
Utilizing the technique of reverse transcription-polymerase chain reaction (RT-PCR), we have examined the expression of transforming growth factor-beta 3 (TGF-β3) by embryonal carcinoma (EC) cells, EC-derived differentiated cells and early mammalian embryos. Using a TGF-β bioassay, we determined that PYS-2 cells express considerable TGF-β activity that cannot be completely neutralized by antibodies specific for TGF-β1 and TGF-β2. We also have determined that PYS-2 cells, as well as F9 EC cells and their differentiated cells, express transcripts for TGF-β3. In addition, we have determined that blastocysts, cultured for three days in serum-containing medium, express TGF-β3 transcripts. Thus, our data suggest that expression of TGF-β3 is initiated during early stages of mammalian development.
Original language | English (US) |
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Pages (from-to) | 1181-1185 |
Number of pages | 5 |
Journal | In Vitro Cellular & Developmental Biology |
Volume | 26 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1990 |
Keywords
- embryonal carcinoma cells
- mammalian embryogenesis
- polymerase chain reaction
- transforming growth factor type-beta
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology