Expression of transforming growth factor-β3 by embryonal carcinoma cells, parietal endoderm-like cells and early mouse embryos

Wendy J. Campbell, David Kelly, Angie Rizzino

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Utilizing the technique of reverse transcription-polymerase chain reaction (RT-PCR), we have examined the expression of transforming growth factor-beta 3 (TGF-β3) by embryonal carcinoma (EC) cells, EC-derived differentiated cells and early mammalian embryos. Using a TGF-β bioassay, we determined that PYS-2 cells express considerable TGF-β activity that cannot be completely neutralized by antibodies specific for TGF-β1 and TGF-β2. We also have determined that PYS-2 cells, as well as F9 EC cells and their differentiated cells, express transcripts for TGF-β3. In addition, we have determined that blastocysts, cultured for three days in serum-containing medium, express TGF-β3 transcripts. Thus, our data suggest that expression of TGF-β3 is initiated during early stages of mammalian development.

Original languageEnglish (US)
Pages (from-to)1181-1185
Number of pages5
JournalIn Vitro Cellular & Developmental Biology
Volume26
Issue number12
DOIs
StatePublished - Dec 1990

Keywords

  • embryonal carcinoma cells
  • mammalian embryogenesis
  • polymerase chain reaction
  • transforming growth factor type-beta

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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