Abstract
Utilizing the technique of reverse transcription-polymerase chain reaction (RT-PCR), we have examined the expression of transforming growth factor-beta 3 (TGF-β3) by embryonal carcinoma (EC) cells, EC-derived differentiated cells and early mammalian embryos. Using a TGF-β bioassay, we determined that PYS-2 cells express considerable TGF-β activity that cannot be completely neutralized by antibodies specific for TGF-β1 and TGF-β2. We also have determined that PYS-2 cells, as well as F9 EC cells and their differentiated cells, express transcripts for TGF-β3. In addition, we have determined that blastocysts, cultured for three days in serum-containing medium, express TGF-β3 transcripts. Thus, our data suggest that expression of TGF-β3 is initiated during early stages of mammalian development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1181-1185 |
| Number of pages | 5 |
| Journal | In Vitro Cellular & Developmental Biology |
| Volume | 26 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1990 |
Keywords
- embryonal carcinoma cells
- mammalian embryogenesis
- polymerase chain reaction
- transforming growth factor type-beta
ASJC Scopus subject areas
- Developmental Biology
- Clinical Biochemistry
- Cell Biology