Expression of UDP-GalNAc:Polypeptide N-acetylgalactosaminyltransferase isozymes T1 and T2 in human colorectal cancer

Takuhiro Kohsaki, Isao Nishimori, Hirofumi Nakayama, Eriko Miyazaki, Hideaki Enzan, Mitsuharu Nomoto, Michael A. Hollingsworth, Saburo Onishi

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Uridine diphosphate (UDP)-GalNAc: polypeptide N-acetylgalactosaminyltransferase (GalNAc transferase) catalyzes the initial step in mucin type O-glycosylation, and its expression has been assumed to be altered between normal epithelial cells and cancer cells. We studied the alteration of GalNAc transferase expression during the carcinogenesis of human colorectal epithelial cells. We produced polyclonal antibodies against synthetic polypeptides with specific sequence to two GalNAc transferase isozymes, T1 and T2. Surgically resected specimens from 50 patients with colorectal cancer were immunohistochemically stained, and the staining grade (percentage of positively stained cells) was compared between cancer and its normal counterpart in the same specimen. Significant signals for both T1 and T2 expression were seen in the supranuclear region of normal and cancer cells, indicating the subcellular localization of the enzymes in the Golgi apparatus. The prevalence of positive staining for T1 and T2 expression in colorectal cancer was significantly higher than that in normal epithelium (P < 0.05). However, the difference in staining grades between cancer and normal tissues varied in each patient. These results indicate that there is variability in the expression patterns of GalNAc transferase isozymes in normal and cancerous cells colorectal among individuals.

Original languageEnglish (US)
Pages (from-to)840-848
Number of pages9
JournalJournal of Gastroenterology
Volume35
Issue number11
DOIs
StatePublished - Dec 23 2000

Keywords

  • Colorectal cancer
  • GalNAc transferase
  • Mucin

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'Expression of UDP-GalNAc:Polypeptide N-acetylgalactosaminyltransferase isozymes T1 and T2 in human colorectal cancer'. Together they form a unique fingerprint.

  • Cite this