Expression profile of osteoblast lineage at defined stages of differentiation

Ivo Kalajzic, Ada Staal, Wen Pin Yang, Yuli Wu, Susan E. Johnson, Jean H.M. Feyen, Winfried Krueger, Peter Maye, Fang Yu, Yifang Zhao, Lynn Kuo, Rishi R. Gupta, Luke E.K. Achenie, Hsin Wei Wang, Dong Guk Shin, David W. Rowe

Research output: Contribution to journalArticlepeer-review

137 Scopus citations


The inherent heterogeneity of bone cells complicates the interpretation of microarray studies designed to identify genes highly associated with osteoblast differentiation. To overcome this problem, we have utilized Col1a1 promoter-green fluorescent protein transgenic mouse lines to isolate bone cells at distinct stages of osteoprogenitor maturation. Comparison of gene expression patterns from unsorted or isolated sorted bone cell populations at days 7 and 17 of calvarial cultures revealed an increased specificity regarding which genes are selectively expressed in a subset of bone cell types during differentiation. Furthermore, distinctly different patterns of gene expression associated with major signaling pathways (Igf1, Bmp, and Wnt) were observed at different levels of maturation. Some of our data differ from current models of osteoprogenitor cell differentiation and emphasize components of the pathways that were not revealed in studies based on a total cell population. Thus, applying methods to generate more homogeneous populations of cells at a defined level of cellular differentiation from a primary osteogenic culture is feasible and leads to a novel interpretation of the gene expression associated with increasing levels of osteoprogenitor maturation.

Original languageEnglish (US)
Pages (from-to)24618-24626
Number of pages9
JournalJournal of Biological Chemistry
Issue number26
StatePublished - Jul 1 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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