Expressions of p53, p63 and PIM1 proteins in nodal or extranodal diffuse large B-cell lymphoma and their prognostic significance

Shu Chen, Xiaoying Xu, Yuanyuan Lei, Pu Huang, Xin Yang, Yanxue Liu, Yuhong Guo, Yanhui Zhang, Yi Pan, Qiongli Zhai, Xianhuo Wang, Lanfang Li, Huilai Zhang, Kai Fu, Bin Meng

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To investigate the correlation between the expression of p53, p63 or PIM1 protein and the survival of patients with nodal or extranodal diffuse large B-cell lymphoma (DLBCL). Methods: Immunohistochemical staining was applied to examine the expressions of p53, p63 and PIM1 proteins in nodal or extranodal DLBCL. The differences in the expressions of p53, p63 and PIM1 proteins between nodal and extranodal DLBCL were analyzed by χ2 test. Kaplan-Meier survival curve and COX proportional hazard model were used to analyze the relationship between the clinicopathological factors (including p53, p63 and PIM1 expressions) and the prognosis of patients with nodal or extranodal DLBCL. Results: Among 212 DLBCL cases, there were 101 nodal cases and 111 extranodal cases (including 55 cases of gastrointestinal DLBCL and 56 cases of non-gastrointestinal DLBCL). The expression rates of p53, p63 and PIM1 proteins in all cases were 19.0%, 25.2% and 54.7%, respectively. Among them, p53 was expressed more frequently in extranodal gastrointestinal cases (P < 0.05), p63 was expressed more commonly in extranodal non-gastrointestinal cases (P < 0.05), whereas PIM1 expression was not significant among the three groups (all P > 0.05). Much more p53-positive cases were observed with international prognostic index (IPI) ≥ 3 (P < 0.05), while p63-positive patients were more common with elevated lactate dehydrogenase (LDH) (P < 0.05). However, PIM1 expression was not significantly correlated with the clinicopathological factors (all P > 0.05). Survival analysis showed that the expression of p53 or p63 protein was a significant inferior prognostic factor for overall survival (OS) and progression-free survival (PFS) in nodal patients or extranodal non-gastrointestinal DLBCL patients, respectively (both P < 0.05), while PIM1 expression was an inferior prognostic factor for PFS in extranodal gastrointestinal cases (P < 0.05). Conclusion: The molecular mechanisms of DLBCL pathogenesis may be different between different locations of DLBCL, which is worth further exploration.

Original languageEnglish (US)
Article number1000-7431 (2019) 11-0931-11
Pages (from-to)931-941
Number of pages11
JournalTumor
Volume39
Issue number11
DOIs
StatePublished - 2019

Keywords

  • Diffuse
  • Immunohistochemistry
  • Large B-cell
  • Location of disease
  • Lymphoma
  • P63 protein
  • PIM1 protein
  • Prognosis
  • Tumor suppressor protein p53

ASJC Scopus subject areas

  • Epidemiology
  • Oncology
  • Cancer Research

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