TY - JOUR
T1 - Extracellular Matrix Secretion Mechanically Reinforces Interlocking Interfaces
AU - McCarthy, Alec
AU - Sharma, Navatha Shree
AU - Holubeck, Phil A.
AU - Brown, Demi
AU - Shah, Rajesh
AU - McGoldrick, Daniel
AU - John, Johnson V.
AU - Shahriar, S. M.Shatil
AU - Xie, Jingwei
N1 - Funding Information:
This work was partially supported by startup funds from the University of Nebraska Medical Center (UNMC), National Institute of General Medical Science (NIGMS), and National Institute of Dental and Craniofacial Research (NIDCR) of the National Institutes of Health under Award Numbers P30GM127200 and R01DE031272, Congressionally Directed Medical Research Program (CDMRP)/Peer Reviewed Medical Research Program (PRMRP) FY19 W81XWH2010207, Nebraska Research Initiative grant, and NE LB606. The authors would like to acknowledge Sedighe Keynia and Wen Qian at the Nebraska NanoEngineering Research Core Facility. The authors would also like to acknowledge Spectro Coating and Claremont Flock for their help in preparing the large quantities of flocked fibers used in this work.
Funding Information:
This work was partially supported by startup funds from the University of Nebraska Medical Center (UNMC), National Institute of General Medical Science (NIGMS), and National Institute of Dental and Craniofacial Research (NIDCR) of the National Institutes of Health under Award Numbers P30GM127200 and R01DE031272, Congressionally Directed Medical Research Program (CDMRP)/Peer Reviewed Medical Research Program (PRMRP) FY19 W81XWH2010207, Nebraska Research Initiative grant, and NE LB606. The authors would like to acknowledge Sedighe Keynia and Wen Qian at the Nebraska NanoEngineering Research Core Facility. The authors would also like to acknowledge Spectro Coating and Claremont Flock for their help in preparing the large quantities of flocked fibers used in this work.
Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2023/2/2
Y1 - 2023/2/2
N2 - Drawing inspiration for biomaterials from biological systems has led to many biomedical innovations. One notable bioinspired device, Velcro, consists of two substrates with interlocking ability. Generating reversibly interlocking biomaterials is an area of investigation, as such devices can allow for modular tissue engineering, reversibly interlocking biomaterial interfaces, or friction-based coupling devices. Here, a biaxially interlocking interface generated using electrostatic flocking is reported. Two electrostatically flocked substrates are mechanically and reversibly interlocked with the ability to resist shearing and compression forces. An initial high-throughput screen of polyamide flock fibers with varying diameters and fiber lengths is conducted to elucidate the roles of different fiber parameters on scaffold mechanical properties. After determining the most desirable parameters via weight scoring, polylactic acid (PLA) fibers are used to emulate the ideal scaffold for in vitro use. PLA flocked scaffolds are populated with osteoblasts and interlocked. Interlocked flocked scaffolds improved cell survivorship under mechanical compression and sustained cell viability and proliferation. Additionally, the compression and shearing resistance of cell-seeded interlocking interfaces increased with increasing extracellular matrix deposition. The introduction of extracellular matrix-reinforced interlocking interfaces may serve as binders for modular tissue engineering, act as scaffolds for engineering tissue interfaces, or enable friction-based couplers for biomedical applications.
AB - Drawing inspiration for biomaterials from biological systems has led to many biomedical innovations. One notable bioinspired device, Velcro, consists of two substrates with interlocking ability. Generating reversibly interlocking biomaterials is an area of investigation, as such devices can allow for modular tissue engineering, reversibly interlocking biomaterial interfaces, or friction-based coupling devices. Here, a biaxially interlocking interface generated using electrostatic flocking is reported. Two electrostatically flocked substrates are mechanically and reversibly interlocked with the ability to resist shearing and compression forces. An initial high-throughput screen of polyamide flock fibers with varying diameters and fiber lengths is conducted to elucidate the roles of different fiber parameters on scaffold mechanical properties. After determining the most desirable parameters via weight scoring, polylactic acid (PLA) fibers are used to emulate the ideal scaffold for in vitro use. PLA flocked scaffolds are populated with osteoblasts and interlocked. Interlocked flocked scaffolds improved cell survivorship under mechanical compression and sustained cell viability and proliferation. Additionally, the compression and shearing resistance of cell-seeded interlocking interfaces increased with increasing extracellular matrix deposition. The introduction of extracellular matrix-reinforced interlocking interfaces may serve as binders for modular tissue engineering, act as scaffolds for engineering tissue interfaces, or enable friction-based couplers for biomedical applications.
KW - compression shielding
KW - electrostatic flocking
KW - extracellular matrix
KW - interlocking interface
KW - mechanical reinforcement
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U2 - 10.1002/adma.202207335
DO - 10.1002/adma.202207335
M3 - Article
C2 - 36444871
AN - SCOPUS:85144300278
SN - 0935-9648
VL - 35
JO - Advanced Materials
JF - Advanced Materials
IS - 5
M1 - 2207335
ER -