Extracellular Vesicle-Mediated Delivery of Ultrasmall Superparamagnetic Iron Oxide Nanoparticles to Mice Brain

Naseer A. Kutchy, Rong Ma, Yutong Liu, Shilpa Buch, Guoku Hu

Research output: Contribution to journalArticlepeer-review

Abstract

Extracellular vesicles (EVs) are small lipid membrane-bound vesicles that can pass the blood–brain barrier. Therefore, EVs could be used for the delivery of therapeutics to the brain. Herein, we investigated the biodistribution of intranasal perfusion of ultrasmall superparamagnetic iron oxide (USPIO)-labeled astrocyte-derived EVs (ADEVs) in mice. We used Western blotting, transmission electron microscopy (TEM), and nanoparticle uptake assay to characterize ADEVs. In addition, intranasal perfusion coupled with magnetic resonance imaging (MRI) was employed to determine the distribution of USPIO-labeled ADEVs in mice. Our results showed the uptake of USPIO by mouse astrocytes and ADEVs. In addition, we confirmed the biodistribution of ADEVs in the brain and other internal organs, including the kidneys, liver, and spleen. Our results suggest that USPIO did not affect mouse astrocyte cell survivability and EV release. Therefore, intranasal delivery of therapeutic loaded EVs could be used for the treatment of various brain disorders.

Original languageEnglish (US)
Article number819516
JournalFrontiers in Pharmacology
Volume13
DOIs
StatePublished - Apr 7 2022

Keywords

  • brain drug delivery
  • extracellular vesicles
  • intranasal perfusion
  • magnetic resonance imaging
  • nanoparticles

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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