Facile Production of Large-Area Cell Arrays Using Surface-Assembled Microdroplets

Karla Perez-Toralla, Angel Olivera-Torres, Mark A. Rose, Amir Monemian Esfahani, Keerthana Reddy, Ruiguo Yang, Stephen A. Morin

Research output: Contribution to journalArticlepeer-review

Abstract

Techniques that enable the spatial arrangement of living cells into defined patterns are broadly applicable to tissue engineering, drug screening, and cell–cell investigations. Achieving large-scale patterning with single-cell resolution while minimizing cell stress/damage is, however, technically challenging using existing methods. Here, a facile and highly scalable technique for the rational design of reconfigurable arrays of cells is reported. Specifically, microdroplets of cell suspensions are assembled using stretchable surface-chemical patterns which, following incubation, yield ordered arrays of cells. The microdroplets are generated using a microfluidic-based aerosol spray nozzle that enables control of the volume/size of the droplets delivered to the surface. Assembly of the cell-loaded microdroplets is achieved via mechanically induced coalescence using substrates with engineered surface-wettability patterns based on extracellular matrices. Robust cell proliferation inside the patterned areas is demonstrated using standard culture techniques. By combining the scalability of aerosol-based delivery and microdroplet surface assembly with user-defined chemical patterns of controlled functionality, the technique reported here provides an innovative methodology for the scalable generation of large-area cell arrays with flexible geometries and tunable resolution.

Original languageEnglish (US)
Article number2000769
JournalAdvanced Science
Volume7
Issue number15
DOIs
StatePublished - Aug 1 2020

Keywords

  • cell arrays
  • microassembly
  • microdroplets
  • surface chemistry
  • tissue engineering

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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