Factors affecting the detection of enteroviruses in cerebrospinal fluid with coxsackievirus B3 and poliovirus 1 cDNA probes

H. A. Rotbart, M. J. Levin, L. P. Villarreal, S. M. Tracy, B. L. Semler, E. Wimmer

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Enteroviruses are common pathogens of meningitis and encephalitis, and infections are often difficult to distinguish clinically from bacterial and herpetic infections of the central nervous system. An array of enteroviruses added to cerebrospinal fluid in reconstruction experiments were detected by a dot hybridization assay. Optimal handling and processing conditions for infected cerebrospinal fluid were established, and the effect on the hybridization reaction of humoral and cellular components of the inflammatory response was determined. Six hybridization probes, derived from poliovirus 1 and coxsackievirus B3, were then tested, singly and in combinations, to optimize the sensitivity and spectrum of the assay. Implications for enteroviral taxonomy based on these experiments are discussed.

Original languageEnglish (US)
Pages (from-to)220-224
Number of pages5
JournalJournal of clinical microbiology
Volume22
Issue number2
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Microbiology (medical)

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