Failure of 2″-deoxycoformycin to protect against transient forebrain ischemia in rat

S. M. Delaney; G. R. Sutherland; J. Peeling; J. D. Geiger

doi:10.1016/0304-3940(93)90340-Q

Failure of 2″-deoxycoformycin to protect against transient forebrain ischemia in rat

S. M. Delaney, G. R. Sutherland, J. Peeling, J. D. Geiger

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

We determined whether 2′-deoxycoformycin (DCF), a potent highly specific inhibitor of adenosine deaminase (ADA), protected against transient forebrain ischemic neuronal injury in rat. Anesthetized male Sprague-Dawley rats received i.p. injections of either saline, 0.5 mg/kg or 5 mg/kg DCF 2 h before undergoing a 10-min forebrain ischemic insult induced by bilateral carotid artery occlusion with concomitant hypotension. Rat brain sections taken 7 days post-ischemia showed damage mostly in the CA1 region of the hippocampus. Quantification of neuronal injury showed no significant differences between saline- or DCF-treated rats. These results indicate that, contrary to previous reports, DCF does not protect against the neuronal damage that follows forebrain ischemia in rat.

Original language	English (US)
Pages (from-to)	31-34
Number of pages	4
Journal	Neuroscience Letters
Volume	149
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(93)90340-Q
State	Published - Jan 4 1993
Externally published	Yes

Keywords

2′-Deoxycoformycin
Adenosine
Adenosine deaminase
Cerebral ischemia
Histopathology

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(93)90340-Q

Cite this

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title = "Failure of 2″-deoxycoformycin to protect against transient forebrain ischemia in rat",

abstract = "We determined whether 2′-deoxycoformycin (DCF), a potent highly specific inhibitor of adenosine deaminase (ADA), protected against transient forebrain ischemic neuronal injury in rat. Anesthetized male Sprague-Dawley rats received i.p. injections of either saline, 0.5 mg/kg or 5 mg/kg DCF 2 h before undergoing a 10-min forebrain ischemic insult induced by bilateral carotid artery occlusion with concomitant hypotension. Rat brain sections taken 7 days post-ischemia showed damage mostly in the CA1 region of the hippocampus. Quantification of neuronal injury showed no significant differences between saline- or DCF-treated rats. These results indicate that, contrary to previous reports, DCF does not protect against the neuronal damage that follows forebrain ischemia in rat.",

keywords = "2′-Deoxycoformycin, Adenosine, Adenosine deaminase, Cerebral ischemia, Histopathology",

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AU - Delaney, S. M.

AU - Sutherland, G. R.

AU - Peeling, J.

AU - Geiger, J. D.

PY - 1993/1/4

Y1 - 1993/1/4

N2 - We determined whether 2′-deoxycoformycin (DCF), a potent highly specific inhibitor of adenosine deaminase (ADA), protected against transient forebrain ischemic neuronal injury in rat. Anesthetized male Sprague-Dawley rats received i.p. injections of either saline, 0.5 mg/kg or 5 mg/kg DCF 2 h before undergoing a 10-min forebrain ischemic insult induced by bilateral carotid artery occlusion with concomitant hypotension. Rat brain sections taken 7 days post-ischemia showed damage mostly in the CA1 region of the hippocampus. Quantification of neuronal injury showed no significant differences between saline- or DCF-treated rats. These results indicate that, contrary to previous reports, DCF does not protect against the neuronal damage that follows forebrain ischemia in rat.

AB - We determined whether 2′-deoxycoformycin (DCF), a potent highly specific inhibitor of adenosine deaminase (ADA), protected against transient forebrain ischemic neuronal injury in rat. Anesthetized male Sprague-Dawley rats received i.p. injections of either saline, 0.5 mg/kg or 5 mg/kg DCF 2 h before undergoing a 10-min forebrain ischemic insult induced by bilateral carotid artery occlusion with concomitant hypotension. Rat brain sections taken 7 days post-ischemia showed damage mostly in the CA1 region of the hippocampus. Quantification of neuronal injury showed no significant differences between saline- or DCF-treated rats. These results indicate that, contrary to previous reports, DCF does not protect against the neuronal damage that follows forebrain ischemia in rat.

KW - 2′-Deoxycoformycin

KW - Adenosine

KW - Adenosine deaminase

KW - Cerebral ischemia

KW - Histopathology

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Failure of 2″-deoxycoformycin to protect against transient forebrain ischemia in rat

Abstract

Keywords

ASJC Scopus subject areas

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