FAN1 acts with FANCI-FANCD2 to promote DNA interstrand cross-link repair

Ting Liu, Gargi Ghosal, Jingsong Yuan, Junjie Chen, Jun Huang

Research output: Contribution to journalArticle

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Abstract

Fanconi anemia (FA) is caused by mutations in 13 Fanc genes and renders cells hypersensitive to DNA interstrand cross-linking (ICL) agents. A central event in the FA pathway is mono-ubiquitylation of the FANCI-FANCD2 (ID) protein complex. Here, we characterize a previously unrecognized nuclease, Fanconi anemia-associated nuclease 1 (FAN1), that promotes ICL repair in a manner strictly dependent on its ability to accumulate at or near sites of DNA damage and that relies on mono-ubiquitylation of the ID complex. Thus, the mono-ubiquitylated ID complex recruits the downstream repair protein FAN1 and facilitates the repair of DNA interstrand cross-links.

Original languageEnglish (US)
Pages (from-to)693-696
Number of pages4
JournalScience
Volume329
Issue number5992
DOIs
StatePublished - Aug 6 2010

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