TY - JOUR
T1 - Fatty acid transport by vectorial acylation in mammals
T2 - Roles played by different isoforms of rat long-chain acyl-CoA synthetases
AU - Tong, Fumin
AU - Black, Paul N.
AU - Coleman, Rosalind A.
AU - DiRusso, Concetta C.
N1 - Funding Information:
The authors thank Ms. Lori Bivins for assistance with the ACSL activity assays. This work was supported by grants from the American Heart Association (AHA 0151215T to CCD and AHA 0315294T, a pre-doctoral fellowship, to FT) and the National Institutes of Health (GM56850 to P.N.B. and C.C.D., and DK59935 to R.A.C.).
PY - 2006/3/1
Y1 - 2006/3/1
N2 - Mammals express multiple isoforms of acyl-CoA synthetase (ACSL1 and ACSL3-6) in various tissues. These enzymes are essential for fatty acid metabolism providing activated intermediates for complex lipid synthesis, protein modification, and β-oxidation. Yeast in contrast express four major ACSLs, which have well-defined functions. Two, Faa1p and Faa4p, are specifically required for fatty acid transport by vectorial acylation. Four ACSLs from the rat were expressed in a yeast faa1Δ faa4Δ strain and their roles in fatty acid transport and trafficking characterized. All four restored ACS activity yet varied in substrate preference. ACSL1, 4, and 6 were able to rescue fatty acid transport activity and triglyceride synthesis. ACSL5, however, was unable to facilitate fatty acid transport despite conferring robust oleoyl-CoA synthetase activity. This is the first study evaluating the role of the mammalian ACSLs in fatty acid transport and supports a role for ACSL1, 4, and 6 in transport by vectorial acylation.
AB - Mammals express multiple isoforms of acyl-CoA synthetase (ACSL1 and ACSL3-6) in various tissues. These enzymes are essential for fatty acid metabolism providing activated intermediates for complex lipid synthesis, protein modification, and β-oxidation. Yeast in contrast express four major ACSLs, which have well-defined functions. Two, Faa1p and Faa4p, are specifically required for fatty acid transport by vectorial acylation. Four ACSLs from the rat were expressed in a yeast faa1Δ faa4Δ strain and their roles in fatty acid transport and trafficking characterized. All four restored ACS activity yet varied in substrate preference. ACSL1, 4, and 6 were able to rescue fatty acid transport activity and triglyceride synthesis. ACSL5, however, was unable to facilitate fatty acid transport despite conferring robust oleoyl-CoA synthetase activity. This is the first study evaluating the role of the mammalian ACSLs in fatty acid transport and supports a role for ACSL1, 4, and 6 in transport by vectorial acylation.
KW - Acyl-CoA synthetase
KW - Fatty acid transport
KW - Rat
KW - Triglyceride synthesis
KW - Vectorial acylation
KW - Yeast
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U2 - 10.1016/j.abb.2006.01.005
DO - 10.1016/j.abb.2006.01.005
M3 - Article
C2 - 16466685
AN - SCOPUS:33644616729
SN - 0003-9861
VL - 447
SP - 46
EP - 52
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -