Feedback effects of horizontal cell membrane potential on cone calcium currents studied with simultaneous recordings

Lucia Cadetti, Wallace B. Thoreson

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Horizontal cell (HC) to cone feedback helps establish the center-surround arrangement of visual receptive fields. It has been shown that HC activity influences cone synaptic output by altering the amplitude and voltage dependence of the calcium current (ICa) in cones. In this study, we obtained voltage-clamp recordings simultaneously from cones and HCs to directly control the membrane potential of HCs and thereby measure the influence of HC membrane potential changes on ICa in adjacent cones. Directly hyperpolarizing voltage clamped HCs produced a negative activation shift and increased the amplitude of ICa in cones. Both of these effects were abolished by enhancing extracellular pH buffering capacity with HEPES. In contrast, addition of the gap junction blocker, carbenoxolone, did not significantly alter the shifts or amplitude changes in cone ICa produced by changes in HC membrane potential. These results support the hypothesis that changes in the HC membrane potential alter the voltage dependence and amplitude of cone ICa by altering extracellular pH levels at the synapse.

Original languageEnglish (US)
Pages (from-to)1992-1995
Number of pages4
JournalJournal of Neurophysiology
Volume95
Issue number3
DOIs
StatePublished - Mar 2006

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

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