To evaluate whether such prostaglandins can alter lung connective tissue secretion by lung fibroblasts, the ability of prostaglandins to modulate the secretion of collagen and fibronectin by HFL-1 cells was determined using an enzyme immunoassay for human type I collagen and for human fibronectin. PGE1 and PGE2 caused a 29 ± 6% reduction in type I collagen secretion but, in contrast, a 39 ± 7% increase in fibronectin secretion (p < 0.01, all comparisons to control). In contrast, PGF(2α), (a prostaglandin which frequently has effects opposite those of PGE1 and PGE2) had no effect on type I collagen secretion (99 ± 7% of control, p > 0.2) bu caused a 20 ± 3% reduction in fibronectin secretion (p < 0.05). Thus fibroblasts are capable of specific modulation of connective macromolecule secretion in response to progstaglandins. Both through the production of prostaglandins and as a target for these mediators, lung fibroblasts play an important role in the cellular response to lung inflammation.
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Critical Care and Intensive Care Medicine
- Cardiology and Cardiovascular Medicine