@article{c370a1fab7544e4782129a2eea380454,
title = "Filamentation involves two overlapping, but Distinct, programs of filamentation in the pathogenic fungus Candida albicans",
abstract = "The ability of the human pathogenic fungus Candida albicans to switch between yeast-like and filamentous forms of growth has long been linked to pathogenesis. Numerous environmental conditions, including growth at high temperatures, nutrient limitation, and exposure to serum, can trigger this morphological switch and are frequently used in in vitro models to identify genes with roles in filamentation. Previous work has suggested that differences exist between the various in vitro models both in the genetic requirements for filamentation and transcriptional responses to distinct filamentation-inducing media, but these differences had not been analyzed in detail. We compared 10 in vitro models for filamentation and found broad genetic and transcriptomic differences between model systems. The comparative analysis enabled the discovery of novel media-independent genetic requirements for filamentation as well as a core filamentation transcriptional profile. Our data also suggest that the physical environment drives distinct programs of filamentation in C. albicans, which has significant implications for filamentation in vivo.",
keywords = "Candida albicans, Comparisons, Filamentation, Hyphal growth, In vitro model",
author = "Jahaun Azadmanesh and Gowen, {Austin M.} and Creger, {Paul E.} and Schafer, {Nichole D.} and Blankenship, {Jill R.}",
note = "Funding Information: We thank the Fungal Genetics Stock Center for the mutant strains analyzed in this work and Jody Franke and Aaron Mitchell for their comments on the manuscript. We also thank the Bioinformatics and Systems Biology Core at University of Nebraska Medical Center for providing RNAseq data analysis services, which receives support from Nebraska Research Initiative (NRI) and National Institutes of Health (NIH) (2P20GM103427 and 5P30CA036727). Bioinformatics analysis was performed at the University of Nebraska Medical Center Bioinformatics Core and RNA sequencing was performed at the University of Nebraska Medical Center Sequencing Core. This work was supported by NIH grant 2P20GM103427 to J.R.B. and a Graduate Research and Creative Activity award from the University of Nebraska at Omaha to P.E.C. The University of Nebraska DNA Sequencing Core receives partial support from the National Institute for General Medical Science (NIGMS) INBRE-P20GM103427-14 and COBRE-1P30GM110768-01 grants as well as The Fred & Pamela Buffett Cancer Center Support Grant-P30CA036727. Publisher Copyright: {\textcopyright} 2017 Azadmanesh et al.",
year = "2017",
month = nov,
day = "1",
doi = "10.1534/g3.117.300224",
language = "English (US)",
volume = "7",
pages = "3797--3808",
journal = "G3 (Bethesda, Md.)",
issn = "2160-1836",
publisher = "Genetics Society of America",
number = "11",
}