Film from peroxidation of an amino phospholipid and its biocompatibility

Hua Jie Wang, Xin Ming Liu, Li Wen Ji, Dong Lan Ma, Qiushi Ren, Jin Ye Wang

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A new kind of phospholipid product with fluorescence was derived from autoxidation of an aminophospholipid, L-α-phosphatidylethanolamine dilinoleoyl in solid film state. Atomic force microscopy showed that the film product was composed of round and elliptical vesicles with diameters of about 20-45 nm. The product was difficult to dissolve in water and most organic solvents. Fourier transform infrared spectroscopy, solid-state 13C NMR, and solid-state 31P NMR indicated that the unsaturated double carbon bond decreased with the reaction, and polymerization within/between phospholipid molecules occurred. Endothelial cells from human vein seeded onto the film showed the highest proliferating activity compared with seeding onto Corning culture plate, glass plate, or phospholipid film with phosphorylcholine head group, as evaluated by 3-(4,5-timethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (p < 0.001). The hemocompatibility of the film was also assessed by platelet adhesion and protein adsorption. The results suggest that the product has potential applications as a new biomaterial coating.

Original languageEnglish (US)
Pages (from-to)411-419
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume79
Issue number2
DOIs
StatePublished - Nov 2006

Keywords

  • Biocompatibility
  • Endothelial cell
  • Fluorescence
  • Oxidation
  • Phospholipid
  • Scanning electron microscope

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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