Filtration of platelet-poor plasma specimens yields platelet-free plasma. Application for batch analysis of beta-thromboglobulin and platelet factor 4

R. C. Schiebout-Clark, M. C. Haven, W. D. Haire, R. B. Wilson, R. S. Markin

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The authors investigated filtration of platelet-poor plasma through a 0.2-μm filter for the analysis of beta-thromboglobulin (β-TG) and platelet factor 4 (PF4) in frozen specimens. Platelets were detected by electron micrograph in the platelet-poor plasma preparation without filtration. No platelets or platelet fragments were seen after filtration. Freezing the unfiltered specimens resulted in a significant increase in the β-TG and PF4 concentrations, presumably because of release of these proteins from the platelet alpha-granules during freezing and thawing. There was no change in the PF4 levels and only a slight decrease in β-TG levels after freezing of the filtered specimens. The filtered samples could be frozen for later batch analysis, resulting in considerable cost savings to the laboratory.

Original languageEnglish (US)
Pages (from-to)206-210
Number of pages5
JournalAmerican journal of clinical pathology
Volume94
Issue number2
DOIs
StatePublished - Jan 1 1990

Keywords

  • Beta-thromboglobulin
  • Filtration
  • Platelet factor 4
  • Platelet-free plasma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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