Abstract
The authors investigated filtration of platelet-poor plasma through a 0.2-μm filter for the analysis of beta-thromboglobulin (β-TG) and platelet factor 4 (PF4) in frozen specimens. Platelets were detected by electron micrograph in the platelet-poor plasma preparation without filtration. No platelets or platelet fragments were seen after filtration. Freezing the unfiltered specimens resulted in a significant increase in the β-TG and PF4 concentrations, presumably because of release of these proteins from the platelet alpha-granules during freezing and thawing. There was no change in the PF4 levels and only a slight decrease in β-TG levels after freezing of the filtered specimens. The filtered samples could be frozen for later batch analysis, resulting in considerable cost savings to the laboratory.
Original language | English (US) |
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Pages (from-to) | 206-210 |
Number of pages | 5 |
Journal | American journal of clinical pathology |
Volume | 94 |
Issue number | 2 |
DOIs | |
State | Published - 1990 |
Keywords
- Beta-thromboglobulin
- Filtration
- Platelet factor 4
- Platelet-free plasma
ASJC Scopus subject areas
- Pathology and Forensic Medicine