Fine genetic mapping and physical delimitation of the lesion mimic gene Spl11 to a 160-kb DNA segment of the rice genome

L. Zeng, Z. Yin, J. Chen, H. Leung, G. L. Wang

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The rice lesion mimic mutant spl11 was previously found to confer broad-spectrum disease resistance to both Magnaporthe grisea and Xanthomonas oryzae pv. oryzae. To better understand the molecular basis underlying cell death and disease resistance in rice, a map-based cloning strategy has been employed to isolate Spl11. Five Spl11-linked RAPD markers were developed and four of them were mapped to rice chromosome 12. A high-resolution genetic map was developed using a segregating population consisting of 1138 lesion mimic individuals. Recombination suppression was observed in the vicinity of Spl11. Three molecular markers tightly linked to Spl11 were identified and used to screen a BAC library. A contig spanning the Spl11 locus was constructed and physical mapping delimited Spl11 to a 160-kb DNA segment within a single BAC clone. These results provide the essential information for the final isolation of this important gene in the rice defense pathway.

Original languageEnglish (US)
Pages (from-to)253-261
Number of pages9
JournalMolecular Genetics and Genomics
Volume268
Issue number2
DOIs
StatePublished - 2002

Keywords

  • BAC contig
  • Cell death
  • Oryza sativa
  • Randomly amplified polymorphic DNA (RAPD)
  • Recombination suppression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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