Fine-tuning interaction between aminoacyl-tRNA synthetase and tRNA for efficient synthesis of proteins containing unnatural amino acids

Nanxi Wang, Tong Ju, Wei Niu, Jiantao Guo

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

By using a directed evolution approach, we have identified aminoacyl-tRNA synthetase variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to the amber nonsense codon in bacteria. We demonstrated that the optimization of anticodon recognition of tRNA by aminoacyl-tRNA synthetase led to improved incorporation efficiency that is unnatural amino acid-specific. The findings will facilitate the creation of an optimized system for the genetic incorporation of unnatural amino acids in bacteria.

Original languageEnglish (US)
Pages (from-to)207-212
Number of pages6
JournalACS Synthetic Biology
Volume4
Issue number3
DOIs
StatePublished - Mar 20 2015

Keywords

  • amber suppression
  • aminoacyl-tRNA synthetase engineering
  • anticodon recognition
  • genetic code expansion
  • unnatural amino acid

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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