Abstract
By using a directed evolution approach, we have identified aminoacyl-tRNA synthetase variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to the amber nonsense codon in bacteria. We demonstrated that the optimization of anticodon recognition of tRNA by aminoacyl-tRNA synthetase led to improved incorporation efficiency that is unnatural amino acid-specific. The findings will facilitate the creation of an optimized system for the genetic incorporation of unnatural amino acids in bacteria.
Original language | English (US) |
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Pages (from-to) | 207-212 |
Number of pages | 6 |
Journal | ACS Synthetic Biology |
Volume | 4 |
Issue number | 3 |
DOIs | |
State | Published - Mar 20 2015 |
Keywords
- amber suppression
- aminoacyl-tRNA synthetase engineering
- anticodon recognition
- genetic code expansion
- unnatural amino acid
ASJC Scopus subject areas
- Biomedical Engineering
- Biochemistry, Genetics and Molecular Biology (miscellaneous)