TY - JOUR
T1 - First description of methyltransferases in extensively drugresistant Klebsiella pneumoniae isolates from Saudi Arabia
AU - Abdalhamid, Baha
AU - Elhadi, Nasreldin
AU - Albunayan, Samar
AU - Alsamman, Khaldoon
AU - Aljindan, Reem
N1 - Publisher Copyright:
© 2017 The Authors.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/7
Y1 - 2017/7
N2 - Purpose. The resistance determinants for carbapenems, fluoroquinolones and aminoglycosides were characterized in 16 extensively drug-resistant Klebsiella pneumoniae (XDRKPN) strains collected from Saudi hospitals during 2014. Methodology. PCR and sequencing were used to detect: blaKPC, blaNDM, blaVIM, blaIMP-1,blaOXA-48, blaCTX-M, blaTEM, blaSHV and ampC for β-lactam resistance; qnrA, qnrB, qnrS, aac(6′)-Ib-cr, qepA and mutations of gyrA and parC for fluoroquinolone resistance; and aacA4, aacC2, aadA1, aphA6, armA and rmtB for aminoglycoside resistance. Enterobacterial repetitive intergenic consensus sequence-based PCR was performed to detect the clonal relatedness. Results. All isolates encoded blaCTX-M, aacC2 and aphA6, together with mutations in gyrA and parC. blaOXA-48, blaNDM-1, aadA1, aacA4, qnrB, aac(6′)-Ib-cr, armA and/or rmtB were detected in different strains. At least 93.2% clonal relatedness was detected among these strains. Conclusions. To our knowledge, this is the first report describing XDRKPN encoding at least seven resistance determinants and harbouring methyltransferases in Saudi Arabia.
AB - Purpose. The resistance determinants for carbapenems, fluoroquinolones and aminoglycosides were characterized in 16 extensively drug-resistant Klebsiella pneumoniae (XDRKPN) strains collected from Saudi hospitals during 2014. Methodology. PCR and sequencing were used to detect: blaKPC, blaNDM, blaVIM, blaIMP-1,blaOXA-48, blaCTX-M, blaTEM, blaSHV and ampC for β-lactam resistance; qnrA, qnrB, qnrS, aac(6′)-Ib-cr, qepA and mutations of gyrA and parC for fluoroquinolone resistance; and aacA4, aacC2, aadA1, aphA6, armA and rmtB for aminoglycoside resistance. Enterobacterial repetitive intergenic consensus sequence-based PCR was performed to detect the clonal relatedness. Results. All isolates encoded blaCTX-M, aacC2 and aphA6, together with mutations in gyrA and parC. blaOXA-48, blaNDM-1, aadA1, aacA4, qnrB, aac(6′)-Ib-cr, armA and/or rmtB were detected in different strains. At least 93.2% clonal relatedness was detected among these strains. Conclusions. To our knowledge, this is the first report describing XDRKPN encoding at least seven resistance determinants and harbouring methyltransferases in Saudi Arabia.
KW - Antimicrobial resistance
KW - Extensive drug resistance
KW - Infection control
KW - Klebsiella pneumoniae
KW - Methyltransferases
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U2 - 10.1099/jmm.0.000480
DO - 10.1099/jmm.0.000480
M3 - Article
C2 - 28639934
AN - SCOPUS:85026654325
SN - 0022-2615
VL - 66
SP - 859
EP - 863
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
IS - 7
M1 - 000480
ER -