TY - JOUR
T1 - First evidence of antibodies against lloviu virus in schreiber’s bent-winged insectivorous bats demonstrate a wide circulation of the virus in spain
AU - De Arellano, Eva Ramírez
AU - Sanchez-Lockhart, Mariano
AU - Perteguer, Maria J.
AU - Bartlett, Maggie
AU - Ortiz, Marta
AU - Campioli, Pamela
AU - Hernández, Ana
AU - Gonzalez, Jeanette
AU - Garcia, Karla
AU - Ramos, Manolo
AU - Jiménez-Clavero, Miguel Ángel
AU - Tenorio, Antonio
AU - Sánchez-Seco, Mª Paz
AU - González, Félix
AU - Echevarría, Juan Emilio
AU - Palacios, Gustavo
AU - Negred, Anabel
N1 - Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/4
Y1 - 2019/4
N2 - Although Lloviu virus (LLOV) was discovered in the carcasses of insectivorous Schreiber’s Bent-winged bats in the caves of Northern Spain in 2002, its infectivity and pathogenicity remain unclear. We examined the seroprevalence of LLOV in potentially exposed Schreiber’s Bent-winged bats (n = 60), common serotine bats (n = 10) as controls, and humans (n = 22) using an immunoblot assay. We found antibodies against LLOV GP2 in all of Schreiber’s Bent-winged bats serum pools, but not in any of the common serotine bats and human pools tested. To confirm this seroreactivity, 52 serums were individually tested using Domain Programmable Arrays (DPA), a phage display based-system serology technique for profiling filovirus epitopes. A serological signature against different LLOV proteins was obtained in 19/52 samples tested (36.5%). The immunodominant response was in the majority specific to LLOV-unique epitopes, confirming that the serological response detected was to LLOV. To our knowledge, this is the first serological evidence of LLOV exposure in live captured Schreiber’s Bent-winged bats, dissociating LLOV circulation as the cause of.
AB - Although Lloviu virus (LLOV) was discovered in the carcasses of insectivorous Schreiber’s Bent-winged bats in the caves of Northern Spain in 2002, its infectivity and pathogenicity remain unclear. We examined the seroprevalence of LLOV in potentially exposed Schreiber’s Bent-winged bats (n = 60), common serotine bats (n = 10) as controls, and humans (n = 22) using an immunoblot assay. We found antibodies against LLOV GP2 in all of Schreiber’s Bent-winged bats serum pools, but not in any of the common serotine bats and human pools tested. To confirm this seroreactivity, 52 serums were individually tested using Domain Programmable Arrays (DPA), a phage display based-system serology technique for profiling filovirus epitopes. A serological signature against different LLOV proteins was obtained in 19/52 samples tested (36.5%). The immunodominant response was in the majority specific to LLOV-unique epitopes, confirming that the serological response detected was to LLOV. To our knowledge, this is the first serological evidence of LLOV exposure in live captured Schreiber’s Bent-winged bats, dissociating LLOV circulation as the cause of.
KW - Bats
KW - Human
KW - Lloviu virus
KW - Prevalence
KW - Serology
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U2 - 10.3390/v11040360
DO - 10.3390/v11040360
M3 - Article
C2 - 31010201
AN - SCOPUS:85065066053
SN - 1999-4915
VL - 11
JO - Viruses
JF - Viruses
IS - 4
M1 - 360
ER -