Flow cytometric detection of neoplastic T cells in patients with mycosis fungoides based on levels of T-cell receptor expression

M. Kuchnio, E. A. Sausville, E. S. Jaffe, T. Greiner, F. M. Foss, J. McClanahan, P. Fukushima, M. A. Stetler-Stevenson

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The authors report the flow cytometric detection of neoplastic T cells in the peripheral blood of four out of five (80%) patients with peripheral blood involvement with mycosis fungoides (Sezary syndrome) based on the levels of T-cell receptor expression as measured by CD3 and TCR-αβ staining. Antigen receptor expression was abnormal in terms of increased density of surface CD3 or TCR-αβ per cell. Other immunophenotypic abnormalities were present in three of these patients. However, in one patient abnormal T-cell receptor expression was the only immunophenotypic evidence of neoplasia, although morphologically abnormal lymphocytes were present and a T-cell clone was detected by polymerase chain reaction (PCR). In another patient, the authors were able to detect development of a new, more aggressive neoplastic T-cell population based on levels of T-cell receptor expression. Levels of T-cell receptor expression may be of diagnostic utility in the evaluation of peripheral blood for the presence of neoplastic T-cell populations.

Original languageEnglish (US)
Pages (from-to)856-860
Number of pages5
JournalAmerican journal of clinical pathology
Volume102
Issue number6
DOIs
StatePublished - Jan 1 1994

Keywords

  • Cutaneous neoplasms
  • Flow cytometry
  • Lymphocyte subsets
  • Malignant lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Kuchnio, M., Sausville, E. A., Jaffe, E. S., Greiner, T., Foss, F. M., McClanahan, J., Fukushima, P., & Stetler-Stevenson, M. A. (1994). Flow cytometric detection of neoplastic T cells in patients with mycosis fungoides based on levels of T-cell receptor expression. American journal of clinical pathology, 102(6), 856-860. https://doi.org/10.1093/ajcp/102.6.856