Fluorescent single-stranded DNA-binding proteins enable in vitro and in vivo studies

Piero R. Bianco, Adam J. Stanenas, Juan Liu, Christopher S. Cohan

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


Fluorescent single-stranded DNA-binding proteins (SSB) that have a defined number of fluorophores per tetramer are invaluable tools to understand biochemical mechanism and biological function. Here, we describe the purification of fluorescent SSB chimeras with a unique number of fluorescent subunits incorporated per tetramer. We describe the use of these tetramers to enable clear visualization of SSB in vivo. Purified chimeras also facilitate single molecule studies (Liu et al., Protein Sci 20:1005-1020, 2011).

Original languageEnglish (US)
Title of host publicationSingle-Stranded DNA Binding Proteins
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages10
ISBN (Print)9781627030311
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • DNA repair
  • DNA replication
  • Fluorescence
  • Fluorescence microscopy
  • Immobilized Metal Affinity Chromatography
  • Recombination
  • SSB

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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