Abstract
A flow injection system for the on-line detection of the intracellular enzyme lactate dehydrogenase (LDH) during fermentation has been developed. The system is comprised of an on-line cell disintegration part, an immobilised dye based expanded bed column for the affinity capture of LDH and a fluorimetric detection unit. The system with a linearity of 0.1-5.4 U LDH ml-1 was applied for the detection of intracellular accumulation of LDH during Lactococcus lactis subsp. lactis cultivation.
Original language | English (US) |
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Pages (from-to) | 1453-1457 |
Number of pages | 5 |
Journal | Biotechnology Letters |
Volume | 22 |
Issue number | 18 |
DOIs | |
State | Published - 2000 |
Externally published | Yes |
Keywords
- Flow injection binding assay
- Intracellular enzyme
- Lactate dehydrogenase
- On-line cell breakage
- On-line monitoring
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology