Formate dehydrogenase in Arabidopsis thaliana: Overexpression and subcellular localization in leaves

Patricia L. Herman, Håkon Ramberg, Renee D. Baack, John Markwell, John C. Osterman

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Formate dehydrogenase (FDH; EC 1.2.1.2) is a NAD-dependent enzyme that catalyzes the oxidation of formate to carbon dioxide in the mitochondria of higher plants. Sequence analyses and other preliminary experiments suggested that FDH might also be targeted to the chloroplasts of Arabidopsis thaliana and other plant species. In the present study, transgenic Arabidopsis and tobacco plants that overexpress Arabidopsis FDH were produced. The FDH specific activity in the leaf tissue of the transgenic plants increased an average of 4.5-fold for Arabidopsis and 31.5-fold for tobacco. Immunodetection and enzyme assays of intact chloroplasts fractionated from the leaves of transgenic tobacco plants suggested that Arabidopsis FDH is present in the chloroplast. Immunogold labeling of Arabidopsis and tobacco detected FDH in both the mitochondria and chloroplasts of the leaf cells. Enzyme assays that were performed to confirm the specificity of Arabidopsis FDH for the NAD cofactor suggested that NADPH was an inhibitor of NAD+ dependent formate oxidation.

Original languageEnglish (US)
Pages (from-to)1137-1145
Number of pages9
JournalPlant Science
Volume163
Issue number6
DOIs
StatePublished - Dec 1 2002

Keywords

  • Arabidopsis
  • Chloroplasts
  • Formate dehydrogenase
  • Mitochondria
  • NADPH
  • Tobacco

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'Formate dehydrogenase in Arabidopsis thaliana: Overexpression and subcellular localization in leaves'. Together they form a unique fingerprint.

  • Cite this