TY - JOUR
T1 - Formation and characterization of lipid droplets of the bovine corpus luteum
AU - Talbott, Heather A.
AU - Plewes, Michele R.
AU - Krause, Crystal
AU - Hou, Xiaoying
AU - Zhang, Pan
AU - Rizzo, William B.
AU - Wood, Jennifer R.
AU - Cupp, Andrea S.
AU - Davis, John S.
N1 - Funding Information:
This work was supported by the Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture (NIFA) [2018-67012-29531 to MRP, 2014-67011-22280 Pre-doctoral award to HT, 2011-67015-20076; 2017-67015-26450 and 2013-67015-20965 to ASC, JRW and JSD]; USDA Hatch grants [NEB26-202/W2112 to ASC, eNEB ANHL 26-213 to ASC and JRW, NEB 26-206 to ASC and JRW]; NIH grants HD087402 and HD092263 to JSD, the VA Nebraska-Western Iowa Health Care System, Department of Veterans Affairs, Office of Research and Development Biomedical Laboratory Research and Development funds [JSD]; and The Olson Center for Women’s Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE [JSD]; National Institute for General Medical Science (NIGMS) [INBRE—P20GM103427-14, COBRE—1P30GM110768-01 to University of Nebraska Microarray Core and the Bioinformatics and Systems Biology Core]; and The Fred & Pamela Buffett Cancer Center Support [P30CA036727 to University of Nebraska Microarray Core and the Bioinformatics and Systems Biology Core].
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Establishment and maintenance of pregnancy depends on progesterone synthesized by luteal tissue in the ovary. Our objective was to identify the characteristics of lipid droplets (LDs) in ovarian steroidogenic cells. We hypothesized that LDs are a major feature of steroidogenic luteal cells and store cholesteryl esters. Whole bovine tissues, isolated ovarian steroidogenic cells (granulosa, theca, small luteal, and large luteal), and isolated luteal LDs were assessed for LD content, LD-associated proteins and lipid analyses. Bovine luteal tissue contained abundant lipid droplets, LD-associated perilipins 2/3/5, hormone-sensitive lipase, and 1-acylglycerol-3-phosphate O-acyltransferase ABHD5. Luteal tissue was enriched in triglycerides (TGs) compared to other tissues, except for adipose tissue. Luteal cells were distinguishable from follicular cells by the presence of LDs, LD-associated proteins, and increased TGs. Furthermore, LDs from large luteal cells were numerous and small; whereas, LDs from small luteal cells were large and less numerous. Isolated LDs contained nearly all of the TGs and cholesteryl esters present in luteal tissue. Isolated luteal LDs were composed primarily of TG, with lesser amounts of cholesteryl esters, diglyceride and other phospholipids. Bovine luteal LDs are distinct from LDs in other bovine tissues, including follicular steroidogenic cells.
AB - Establishment and maintenance of pregnancy depends on progesterone synthesized by luteal tissue in the ovary. Our objective was to identify the characteristics of lipid droplets (LDs) in ovarian steroidogenic cells. We hypothesized that LDs are a major feature of steroidogenic luteal cells and store cholesteryl esters. Whole bovine tissues, isolated ovarian steroidogenic cells (granulosa, theca, small luteal, and large luteal), and isolated luteal LDs were assessed for LD content, LD-associated proteins and lipid analyses. Bovine luteal tissue contained abundant lipid droplets, LD-associated perilipins 2/3/5, hormone-sensitive lipase, and 1-acylglycerol-3-phosphate O-acyltransferase ABHD5. Luteal tissue was enriched in triglycerides (TGs) compared to other tissues, except for adipose tissue. Luteal cells were distinguishable from follicular cells by the presence of LDs, LD-associated proteins, and increased TGs. Furthermore, LDs from large luteal cells were numerous and small; whereas, LDs from small luteal cells were large and less numerous. Isolated LDs contained nearly all of the TGs and cholesteryl esters present in luteal tissue. Isolated luteal LDs were composed primarily of TG, with lesser amounts of cholesteryl esters, diglyceride and other phospholipids. Bovine luteal LDs are distinct from LDs in other bovine tissues, including follicular steroidogenic cells.
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U2 - 10.1038/s41598-020-68091-2
DO - 10.1038/s41598-020-68091-2
M3 - Article
C2 - 32647143
AN - SCOPUS:85087312550
VL - 10
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 11287
ER -