TY - JOUR
T1 - Fractionation of Aloe vera L. inner gel, purification and molecular profiling of activity
AU - Talmadge, James
AU - Chavez, Jennifer
AU - Jacobs, Lori
AU - Munger, Corey
AU - Chinnah, Tony
AU - Chow, Jimmy T.
AU - Williamson, David
AU - Yates, Kenneth
N1 - Funding Information:
This research was supported in part by a contract with Carrington Laboratories, Irving, TX, USA.
PY - 2004/12/20
Y1 - 2004/12/20
N2 - Products derived from the inner gel of the Aloe vera L. plant have demonstrated multiple clinical activities, and are used routinely to accelerate wound healing. However, typical of natural products, the complex nature of Aloe vera gels may contribute to diverse pharmacologic activities. Our focus on the hematopoietic activities of Aloe vera extracts is extended by these functional studies, which used purified fractions from Aloe vera gel and included a preliminary organ-specific in vitro molecular profile. Studies using a >99% pure carbohydrate fraction from Aloe vera extracts revealed increased hematopoietic and hematologic activity compared to the starting material. In addition, this fraction differentially regulated liver and lung cytokine mRNA levels, resulting in significant increases in message for hematopoietic cytokines [granulocyte colony stimulating factor (G-CSF) and stem cell factor (SCF)]. This profile of activity differed from another fraction obtained from Aloe vera, suggesting the potential for diverse pharmacologic activity. The molecular studies were undertaken using co-cultures of organ slices to limit the amount of purified material required. In summary, these studies revealed significant hematopoietic activity by both pharmacologic and molecular analysis using a >99% pure carbohydrate fraction from Aloe vera gels.
AB - Products derived from the inner gel of the Aloe vera L. plant have demonstrated multiple clinical activities, and are used routinely to accelerate wound healing. However, typical of natural products, the complex nature of Aloe vera gels may contribute to diverse pharmacologic activities. Our focus on the hematopoietic activities of Aloe vera extracts is extended by these functional studies, which used purified fractions from Aloe vera gel and included a preliminary organ-specific in vitro molecular profile. Studies using a >99% pure carbohydrate fraction from Aloe vera extracts revealed increased hematopoietic and hematologic activity compared to the starting material. In addition, this fraction differentially regulated liver and lung cytokine mRNA levels, resulting in significant increases in message for hematopoietic cytokines [granulocyte colony stimulating factor (G-CSF) and stem cell factor (SCF)]. This profile of activity differed from another fraction obtained from Aloe vera, suggesting the potential for diverse pharmacologic activity. The molecular studies were undertaken using co-cultures of organ slices to limit the amount of purified material required. In summary, these studies revealed significant hematopoietic activity by both pharmacologic and molecular analysis using a >99% pure carbohydrate fraction from Aloe vera gels.
KW - Aloe vera
KW - Fractionation
KW - Purification
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U2 - 10.1016/j.intimp.2004.07.013
DO - 10.1016/j.intimp.2004.07.013
M3 - Article
C2 - 15531292
AN - SCOPUS:8644273179
VL - 4
SP - 1757
EP - 1773
JO - International Immunopharmacology
JF - International Immunopharmacology
SN - 1567-5769
IS - 14 SPEC.ISS.
ER -